小麦幼苗对涝胁迫的生理响应实验研究分析 摘 要：以“周麦18”为实验材料，利用室内水培实验，研究了不同历时水涝胁迫对小麦幼苗生长发育及生理生化变化的影响。测定了叶绿素﹑丙二醛和游离脯氨酸含量以及超氧化物歧化酶﹑过氧化物酶、过氧化氢酶活性。结果如下：随着涝胁迫历时的增长，小麦幼苗叶片的叶绿素含量逐渐降低，而MDA含量和游离脯氨酸含量上升；超氧化物歧化酶﹑过氧化物酶和过氧化氢酶活性呈现先升高后降低的趋势。这些结果表明：随着涝胁迫历时的增长，小麦幼苗的生长损害程度越大。
关键词：涝胁迫；小麦幼苗；生理响应
Physiological Responses of Wheat Seedling to Waterlogging Stress
Abstract: Using "zhoumai18" as the experimental material, effects of waterlogging stress for different duration on seedling growth and physiological and biochemical changes of wheat seeding was studied by indoor hydroponic culture. The content of chlorophyll, malondialdehyde(MDA), free proline and activity of superoxide dismutase (SOD), peroxidase (POD), catalase activity (CAT) were measured. The results obtained as follows: with the increase of waterlogging stress duration, chlorophyll content of wheat seedling leaves was gradually reduced, while the MDA content and free proline conten twere increased; Superoxide dismutase(SOD), peroxidase (POD) and catalase activity (CAT) rised at the initial stage and then dropped at the following stage. These results indicated that: wheat seedlings were greatly damaged with the diachronic waterlogging stress.
Key Words: Waterlogging stress; Wheat seedlings; Physiological responses
目 录
摘要 1
Abstract 1
引言 1
1材料与方法 2
1.1供试材料 2
1.2材料培养与处理 2
1.3生理指标测定方法 2
2结果与分析 3
2.1涝胁迫对小麦幼苗叶片内叶绿素含量的影响 3
2.2涝胁迫对小麦幼苗叶片内SOD活性的影响 3
2.3涝胁迫对小麦幼苗叶片内MDA含量的影响 4
2.4 涝胁迫对小麦幼苗叶片内POD活性的影响 5
2.5涝胁迫对小麦幼苗叶片内CAT活性的影响 5
2.6涝胁迫对小麦幼苗叶片内脯氨酸含量的影响 6
3结论与讨论 7
参考文献 8
致谢 10，3255