本文研究铜萃取改质剂壬基酚对嗜酸氧化亚铁钩端螺旋菌(Leptospirillumferrooxidans，简称L.f菌)以及黄铜矿和黄铁矿表面性质的影响，以及在不同壬基酚体积浓度、不同污染途径下对细菌生长、在矿石表面吸附的影响。结果表明，随着污染程度（污染程度：净菌+污基污菌+净基净菌+净基）的加深，L.f菌生长速度降低，L.f菌的氧化活性逐渐减小，同时细胞峰值浓度降低，吸附平衡时间缩短，吸附速度越快，平衡时吸附率越高。同种污染途径下，随着壬基酚体积浓度的提高，细菌生长代谢受到的抑制作用增强。经过有机相污染后，细菌与矿石疏水性加强的表现有利于吸附，加快了吸附速度，细菌与矿石之间表现为静电吸引力并且吸引力随着污染程度的加深而增强，加快了污染发生时L.f菌与黄铁矿之间的吸附速度。39032
毕业论文关键字：溶剂萃取；嗜酸氧化亚铁钩端螺旋菌；壬基酚；黄铜矿；吸附；表面性质
Abstract
The impact ofchalcopyriteandpyritesurface propertiesof Copperextractionmodifiernonylphenolethyleneoxidepaperacidophilushookendpylori(Leptospirillumferrooxidans,calledLfbacteria), as well asindifferentvolume concentration ofnonylphenol, differentbacterialcontaminationpathway onthe growth,themineralsurface adsorption. The resultsshowed that withthe degree of pollution(pollution degree: Net bacteria+dirtygroupsewagebacteria+bacteria+net basenetnetbasis)deepened, Lf reducebacteriagrowth, oxidative activityLfbacteriadecreased,whilecellpeakconcentration decreased, the adsorptionequilibriumtime is shortened, the faster theabsorption, the higher therate ofadsorptionequilibrium. The same kind ofpollutionunderway, with increasingvolume concentrationofnonylphenol, inhibition ofbacterial growth andmetabolism has beenincreased.After theorganicphasepollution, bacteria andmineralsto strengthenthe performance ofhydrophobicadsorptionis conduciveto speed up theabsorptionrate. Withthe deepening ofthe attractivenessof pollutionandenhanceand speed up theabsorptionrateLfbetweenbacteriaandpyritecontamination occurswhenbacteria andmineralsbetweenthe performanceandthe electrostatic attraction.
KeyWords：Solvent extraction; Leptospirillumferrooxidans；nonylphenol; chalcopyrite；adsorption; surface properties
目录
摘要    1
Abstract    1
目录    2
1文献综述    4
1.1 浸矿微生物种类    4
1.2 微生物在矿石表面的吸附    4
1.2.1 细菌表面性质对吸附影响    5
1.2.2 矿物表面性质对细菌吸附的影响    6
1.3 细菌浸出机理    7
1.4 铜萃取时萃余液中有机物的夹带和溶解    9
1.5 研究的目的和意义    9
1.6 研究内容    10
2实验方案设计    10
2.1 实验原料    10
2.2 实验方法    10
2.2.1 矿石的预处理    10
2.2.2 萃余液夹带和有机污染的模拟（分别对细菌、MAC培养基进行有机相污染）    11
2.2.3 细菌培养    11
2.2.4 细菌在矿石表面的吸附    11
2.2.5 细菌扩大培养    12
2.3性能表征方法    12
2.3.1细菌计数    12
2.3.2接触角的测定    12
2.3.4 Zeta电位的测定    13
2.3.5pH的测定    13
2.3.6 Fe2+浓度测定方法    14
3结果与讨论    14
3.1 有机物黄铁矿与黄铜矿表面性质的影响    14
3.1.1 有机物对矿石表面接触角的影响    14
3.1.2矿石表面的Zeta电位    14 铜萃取改质剂对浸矿细菌和矿石表面性质的影响:http://www.751com.cn/huaxue/lunwen_38131.html