pMD19-T- 5333    pMD19-T derivative carrying MXAN_5333    This study
pMD19-T- 6908    pMD19-T derivative carrying MXAN_6908    This study
pBJ113    Derived from PUC118 with galK and Kanr    Yang Zhaoming and Mao Xiaohua
pBJ113-2921    pBJ113 derivative carrying MXAN_2921    This study
pBJ113-3506    pBJ113 derivative carrying MXAN_3506    This study
pBJ113-4613    pBJ113 derivative carrying MXAN_4613    This study
pBJ113-5333    pBJ113 derivative carrying MXAN_5333    This study
pBJ113-6908    pBJ113 derivative carrying MXAN_6908    This study
1．2  引物
表2-1-2实验所用引物
Table 2-1-2 Oligonucleotide primers used in the experiment
引物    序列 (5'-3')    设计目的
2921F    CCCAAGCTTGTCCTTGATGCCCTCTGCG    Forward primer for MXAN_2921 homologous fragment
2921R    CCGGAATTCAAGCCTGCTTCCCATTCGTC    Reverse primer for MXAN_2921 homologous fragment
3506F    CCCAAGCTTGAAGCGGGTAGCGGTCCT    Forward primer for MXAN_3506 homologous fragment
3506R    CCGGAATTCGGAACCACGCAATGGTCTC    Reverse primer for MXAN_3506 homologous fragment
4613F    CCCAAGCTTGGTGGCTGTGGCTTCATCG    Forward primer for MXAN_4613 homologous fragment
4613R    CCGGAATTCCGCGTTCGGTACTGCGTTTC    Reverse primer for MXAN_4613 homologous fragment
5333F    CCCAAGCTTGGCATCGTCTTCCACAACATCA    Forward primer for MXAN_5333 homologous fragment
5333R    CCGGAATTCTTCACGCAGCCGCTCGTT    Reverse primer for MXAN_5333 homologous fragment
6908F    CCCAAGCTTCCGAGCGAGAACTGTGGGAG    Forward primer for MXAN_6908 homologous fragment
6908R    CCGGAATTCTGGAGCCCTTGTTGCCGTA    Reverse primer for MXAN_6908 homologous fragment
2921tF    TGCCCTGGTCCACGATTG    Forward primer for identifying
△MXAN_2921
2921tR    TCTGGCAACACCTCCCCC    Reverse primer for identifying
△MXAN_2921
3506tF    ACGTTCGACGAGCTGTGAGAG    Forward primer for identifying
△MXAN_3506
3506tR    GACCTTCACGCAACCTCCC    Reverse primer for identifying
△MXAN_3506
4613tF    TGGAGCAAGGAACACACGC    Forward primer for identifying
△MXAN_4613
4613tR    GTGGACCCCTTCGATACGC    Reverse primer for identifying
△MXAN_4613
5333tF    CCCGGTGACCTGGTCCTCT    Forward primer for identifying
△MXAN_5333
5333tR    TGGGCGCGTAGAGGTAGGT    Reverse primer for identifying
△MXAN_5333
6908tF    GCGAAAACTGCCAGGAGGA    Forward primer for identifying
△MXAN_6908
6908tR    CGATGAAGGTGGCGAAGAA    Reverse primer for identifying
△MXAN_6908
2921exF    TCCCCGGCATCCACGCGCGCTA    Forward primer for testing the expression of MXAN_2921
2921exR    GTTCCGAAGCCTGGATGCGCA    Reverse primer for testing the expression of MXAN_2921
3506exF    GGCCTCGCCGGCCTCGCCCA    Forward primer for testing the expression of MXAN_ 3506
3506exR    GTCTACGACGAGGCCAAGCG    Reverse primer for testing the expression of MXAN_3506
4613exF    CGCCGGCCCGGCCCTTCTCCA    Forward primer for testing the expression of MXAN_4613 多糖合成途径中功能基因的鉴定及其在粘细菌发育中的研究(4):http://www.751com.cn/shengwu/lunwen_19366.html