猪卵母细胞IVM和精子质量对胚胎发育影响的研究

摘要：本实验采用卵母细胞体外成熟、精子获能、体外受精及胚胎培养技术，通过对照重复试验来探讨猪精子不同的获能方法、精子活率及卵母细胞的选择对猪体外受精后卵裂率和胚胎发育的影响。结果表明，同一猪种精液采用不同的获能液，体外受精后卵裂率无显著差异（P＞0.05），但各组的桑葚胚率，三元猪+获能液A组明显高于三元猪+获能液B组（P＜0.05），而新淮猪的两组情况相反；精子活率的三组实验的卵裂率和桑葚胚率差异显著（P＜0.05），且随精子活率的降低而降低；体外成熟前卵母细胞外的卵丘细胞层数＜3层，体外受精后的卵裂率和桑葚胚率都明显低于卵丘细胞层数≥3层的卵母细胞（P＜0.05）。25685
毕业论文关键词：卵母细胞；精子获能；体外受精
The study of the effects of oocytes maturation and sperm quality on the developmental viability of pig embryos
Abstract：In the study,techniques including in vitro maturation of oocytes,capacitation of sperm ,in vitro fertilization and embryo culture were used to research the effects of different methods of capacitation of sperm, sperm viability and selection of oocytes on cleavage rate and embryo development .Results show that no significant difference in the cleavage rate of the same pig semen with different emollients(P>0.05),the morula rate was significantly higher in the Sanyuan pig+Capacitation fluid A than in the Sanyuan pig+Capacitation fluid B(P<0.05).Subsequently, semina from Xinhuai with different sperm viability were performed for the IVF. And results show that significant differences in cleavage rate and morula rate were observed in fertilized embryos between different sperm viability(P<0.05).Finally,the cleavage rate and morula rate in the COCs with less than 3 layers were significantly lower than in the COCs with more than 3 layers(P<0.05).
Key words: Oocytes;capacitation of sperm;in vitro fertilization
目录

摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法2
1.1材料2
1.1.1仪器设备2
1.1.2卵母细胞、精液及相关试剂2
1.2溶液的配制2
1.2.1获能液A2
1.2.2获能液B2
1.3方法2
1.3.1卵母细胞的采集和体外成熟2
1.3.2成熟卵母细胞的处理3
1.3.3鲜精的处理和质量检测3
1.3.4体外受精和胚胎培养3
1.3.5观察多精入卵3
1.4统计与分析3
2结果与分析3
2.1不同猪种及获能液对胚胎发育的影响3
2.2精子获能前活率对胚胎发育的影响4
2.3卵丘-卵母细胞复合体的卵丘细胞层数对胚胎发育的影响4
3讨论 5
3.1不同猪种及获能液对胚胎发育的影响5
3.2精子获能前活率对胚胎发育的影响5
3.3卵丘-卵母细胞复合体的卵丘细胞层数对胚胎发育的影响6
致谢6
参考文献7
猪卵母细胞IVM和精子质量对胚胎发育影响的研究
引言猪的胚胎工程已成为推动畜牧业发展不可或缺的一大研究领域，通过其可以生产转基因猪或基因组修饰猪，可以改良品种、培育抗病猪种、提高肉质。同时，因为猪的基因与人相似度高，所以猪的胚胎工程在医学界备受欢迎，能够制备人类疾病模型、进行器官移植、生产药物蛋白、研究发育机理等。为了提供大量优质的猪卵母细胞和胚胎，如何完善猪的体外繁殖技术成为研究重点。
在国外，猪体外受精后的囊胚发育率为20%，有的会达到44.4%[1]，目前国内猪卵母细胞体外受精后的卵裂率达40%以上[2]，但是关于囊胚发育率的研究统计很少报道。与牛羊的胚胎体外生产相比，国内外猪的胚胎体外生产效率太低，技术也不够成熟。而选择优质的成熟卵母细胞和精子是提高胚胎体外生产效率的途径之一。猪卵母细胞IVM和精子质量对胚胎发育影响的研究:http://www.751com.cn/shengwu/lunwen_19569.html