摘要：花青苷是一种水溶性色素，广泛存在于植物的体内，是非常有潜力的天然色素的来源。因资源丰富、颜色鲜艳、无毒安全和具有抗氧化性等优势，花青苷的研究和开发利用备受关注。6-BA是一种人工合成的细胞分裂素，不但在细胞生长和分化及其他相关的生理活动过程中起重要作用，还可以促进花青苷的积累，抑制或促进相关酶的活性。本实验以不结球白菜紫色品种NJX1-4和绿色品种苏州青为材料，利用外源6-BA处理植株，并测定不同时间点叶片中花青苷含量，抗氧化性及相关酶活性，探索6-BA对不结球白菜中花青苷的抗氧化性及相关酶活性的调控作用，为今后进一步研究花青苷的功能机制提供理论基础。26233
毕业论文关键词：花青苷；6-BA；抗氧化性；酶活性，不结球白菜
Effect of exogenous 6-BA on anthocyanins antioxidant activity and related enzyme activities in Purple non-heading Chinese Cabbage
Student majoring in Protected agriculture science and engineering    Chula Sa
 Tutor    Chunmei Hu
Abstract： Anthocyanins are water-soluble pigments which are widely found in plants, it is a potential source of natural pigments. The research and development of anthocyanins are important for its rich resources, bright colors, safe and antioxidant activity.6-BA is a synthetic cytokinin. It plays an important role in the cell growth and differentiation and other related physiological activities of the process. In addition, 6-BA can promote the accumulation of anthocyanins,　as well as inhibit or induce other enzyme activity. In this study, we used two self-line of non-heading Chinese Cabbage: purple leaves NJX1-4 and green suzhouqing as the experimental materials. Leaves were treaded by exogenous 6-BA to explored the effect on anthocyanins antioxidant activity and related enzyme activities, providing a theoretical basis for further study of the functional mechanism of anthocyanins.
Key words: Anthocyanins; 6-BA; Antioxidant activity; Enzyme activity; non-heading Chinese Cabbage
目  录
摘要    3
关键词    3
Abstract    3
Key words    3
1  材料与方法    4
1．1  试验材料    5
1．2  试验方法    5
1．2．1  外源6-BA处理    5
1．2．2  总花青苷含量测定    5
1．2．3  总酚含量测定    5
1．2．4  抗氧化酶活性测定    5
1．2．5  叶片花青苷的抗氧化活性分析    6
1．3  数据分析    6
2  结果与分析    6
2．1  外源6-BA对不结球白菜中花青苷含量的影响    6
2．2  外源6-BA对不结球白菜叶片中总酚含量的影响    7
2．3  外源6-BA对不结球白菜叶片中相关酶活性的影响    8
2．3．1  外源6-BA处理对紫色不结球白菜中超氧化物歧化酶（SOD）活性的影响    8
2．3．2  外源6-BA处理对紫色不结球白菜中过氧化物酶（POD）活性的影响    8
2．3．3  源6-BA处理对紫色不结球白菜中过氧化氢酶（CAT）活性的影响    9
2．4  叶片抗氧化活性测定    9
3  讨论    10
3．1  外源6-BA处理不结球白菜后总花青苷含量和总酚及抗氧化能力的比较    10
3．2  外源6-BA处理对不结球紫色白菜中抗氧化酶的影响    10
3．3  外源6-BA处理对不结球紫色白菜中花青苷的抗氧化性影响    11 外源6-BA对不结球紫色白菜中花青苷抗氧化性及相关酶活性的调控作用:http://www.751com.cn/shengwu/lunwen_20337.html