薰衣草精油微乳液的制备及抑菌研究

摘要：本文利用微乳化技术，用拟三元相图法制备以薰衣草精油为油相的微乳体
系，研究蔗糖脂肪酸酯（ SE16 ）、双琥珀酸酯磺酸钠（ AOT ）、吐温 40 或吐温 8 0
作为表面活性剂，乙醇和丙二醇作为助表面活性剂对精油微乳体系相行为的影
响，观察微乳液对化妆品常见腐败菌铜绿假单胞菌、金黄色葡萄球菌和黑曲霉的
抑制效果。实验结果表明：油相中加入短链醇、水相中加入丙二醇相对于单一的
油相、水相更容易形成微乳体系，且随着油相中乙醇浓度的增大、水相中丙二醇
浓度的增大，薰衣草精油微乳体系单相区面积也相应的增大，混合的表面活性剂
效果也优于单一的表面活性剂，选取配方为薰衣草精油 : 乙醇 : 吐温
80 : AOT =7:7:3:3 进行抑菌效果研究。薰衣草精油微乳体系对金黄色葡萄球菌和
黑曲霉最低抑菌浓度分别为 625 μ L/L 和 1250 μ L/L ，对铜绿假单胞菌未检出有抑
制效果。5278
关键词：薰衣草精油；微乳液；抑菌
Formation Formation Formation Formation and and and and Antimicrobial Antimicrobial Antimicrobial Antimicrobial Activity Activity Activity Activity of of of of Lavender Lavender Lavender Lavender
essential essential essential essential oil oil oil oil microemulsion microemulsion microemulsion microemulsion
Abstract: Abstract: Abstract: Abstract: Microemulsion technology and pseudo ternary phase diagram was used to
produce microemulsion system. Lavender essential oil was oil, SE16 ， AOT, Tween-40,
Tween-80 were treated as surfactant and ethanol, propylene glycol were treated as
cosurfactan. The antimicrobial activity of the microemulsion against Pseudomonas
aeruginosa , Staphylococcus aureus , Aspergillus niger which usually pollute cosmetic
was also studied. The oil solubilization was improved in the presence of ethanol and
propylene glycol. The function of mixed surfactant was better than single one. The
formulation of LEO, ethanol, Tween-80 and AOT in the mass ratios of 7:7:3:3 was
selected for the antimicrobial assay. The minimum inhibition concentrations (MIC) of
the microemulsion against S. aureus and A . niger were were respectively 625 μ L/L and
1250 μ L/L.
Keywords: Keywords: Keywords: Keywords: Lavender essential oil ； microemulsion ； Antimicrobial Activity目录
1 前言 1
1.1 植物精油概述 1
1.1.1 植物精油的化学成分及其功效 . 1
1.1.2 植物精油在化妆品中的应用 . 1
1.1.3 植物精油的抑菌特性 . 2
1.1.4 薰衣草精油简介 . 2
1.2 微乳液概述 4
1.3 植物精油微乳液的研究概况 4
1.4 本课题的研究目的和意义 5
2 实验材料和方法 7
2.1 实验材料 7
2.1.1 实验试剂： . 7
2.1.2 植物精油： . 7
2.1.3 菌种及培养基： . 7
2.1.4 主要仪器及设备： . 8
2.2 实验方法 8
2.2.1 拟三元相图构建微乳体系 . 8
2.2.2 微乳液的物理稳定性 . 8
2.2.3 薰衣草精油微乳液对微生物的抑制效果 . 9
3 结果与讨论 .. 10
3.1 短链醇对微乳体系相行为的影响 .. 10
3.1.1 不同链长的短链醇对微乳体系相行为的影响 10
3.1.2 短链醇与薰衣草精油的比例对微乳体系相行为的影响 ... 11
3.2 助溶剂对微乳体系相行为的影响 .. 13
3.3 表面活性剂对微乳体系相行为的影响 .. 14
3.3.1 表面活性剂种类对微乳体系相行为的影响 ... 14
3.3.2 混合表面活性剂对微乳体系相行为的影响 ... 15
3.4 微乳配方的确定 .. 17
3.5 薰衣草微乳液对微生物的抑制效果 .. 17
3.5.1 微乳液对铜绿假单胞杆菌和金黄色葡萄球菌的最低抑菌浓度 17 薰衣草精油微乳液的制备及抑菌研究:http://www.751com.cn/shengwu/lunwen_2247.html