中华鳖白点病致病菌的筛选与鉴定

摘要中华鳖（Pelodiscus sinensis）属于爬行纲、龟鳖目、鳖科、鳖属，是一种淡水资源名品，该种生物常生活在河流、湖泊、沼泽等一系列水流缓和而鱼虾资源相对丰富的淡水水域中。中华鳖有很高的食用和药用价值，所以中华鳖的市场随着人民的生活水平的提高而持续不断的扩大，鳖业的养殖成为了农村发展经济的手段，成了各个省市重点的产业扶持对象。但是在中华鳖的工厂化养殖过程中由于商品鳖交易频繁、人工养殖密度过大和养殖环境污染等各种各样的因素，各类病害在养殖群体中频繁爆发。根据已经有的文献记录，我们可以发现中华鳖疾病现已经达到了30余种。本文重在研究浙江省中华鳖养殖过程中常见细菌性疾病-白点病，白点病的死亡率极高。本文旨在对中华鳖的白点病致病菌进行筛选和鉴定进行研究，希望相关研究结果能为研究中华鳖白点病的防治提供理论依据，对将来更好的养殖中华鳖提供理论依据。44068

Abstract Lumen (Pelodiscus sinensis) belongs to crawl outline, chelonia, turtle. turtle, is a famous freshwater resources, which are often live in rivers, lakes, swamps, and a series of freshwater,where are relatively rich resources of Fish and shrimp.Lumen has high edible and medicinal value, so the the market of lumenn ,with the improvement of people's living standard ,continu to expand. The turtle inheritance breeding has become a means of rural economic development, a key industrial object supported by various provinces and cities.But in the process of the lumen of factory farming, due to the large commodity turtle trading frequency, artificial breeding density and breeding a variety of factors ,such as environmental pollution, various disease broke out frequently in community.According to existing literature records, we can find that the disease have reached more than 30.This paper studies Chinese turtle’s bacterial disease - white spot,zhejiang province,.white spot have high mortality rate.The purpose of this paper is to screening and identificat the pathogenic bacteria of lumen ‘s white spot disease ,I hope this research results can provide theoretical basis for disease prevention and control the lumen’s white spot disease, and provide the theory basis for the better future breeding lumen.

毕业论文关键词：中华鳖；嗜水气单胞菌；筛选；鉴定

Key word: Pelodiscus sinensis; A.hydrophila; Selection; identification

1 引言 1

1.1中华鳖的生活习性 1

1.2中华鳖致病的研究进展和疾病种类 1

2 嗜水气单胞菌 2

2.1嗜水气单胞菌的生物介绍 2

2.2嗜水气单胞菌的致病性 3

2.3嗜水气单胞菌的致病机理 3

2.3.1溶血素 3

2.3.2肠毒素 4

2.3.3胞外蛋白酶 4

2.3.4感染嗜水气单胞菌的现象 4

3中华鳖养殖病虫害的防治 4

4材料和方法 5

4.1实验材料 5

4.1.1实验动物 5

4.1.2营养琼脂培养基配制 5

4.1.3设备仪器 5

4.1.4试剂 5

4.2方法中华鳖白点病致病菌的筛选与鉴定:http://www.751com.cn/shengwu/lunwen_45234.html