杭白菊的快速繁殖及茎尖脱毒培养

摘要选取杭白菊（Chrysanthemum morifolium Ramat）的嫩芽为外植体，接种在诱导芽分化培养基（MS+0.5mg/L 6-BA）中进行培养，23d左右，植株生长旺盛，再将外植体接种于愈伤组织诱导培养基（MS+0.2mg/L NAA +2mg/L 6-BA）中进行继代培养，最后将部分芽接种到生根培养基(1/2MS+0.2mg/L NAA)中培养，15d生根，平均长根数6.63，平均根长3.96cm，21d后进行移栽，移栽后存活率为87.5%。另一部分继代培养的外植体，进行茎尖脱毒，然后在茎尖培养基（MS+0.2mg/L 6-BA）中进行培养，一周后，茎尖开始变绿；13d后，开始出现愈伤组织；28d后愈伤组织变大，有些开始出芽，三个月后菊花茎尖出现褐化，但没有采取措施导致死亡。实验发现，培养基MS+0.1mg/L NAA +2mg/L 6-BA比较适合丛生芽增殖，平均增殖倍数可达4.9倍；培养基MS+1 mg/L NAA +0.1mg/L 6-BA比较有利于杭白菊根的诱导，每瓶平均长根数5.35根；培养基MS+0.1mg/L NAA +1mg/L 6-BA最适合杭白菊的愈伤组织分化以及芽诱导，芽诱导率有50%。44214

Abstract Select Chrysanthemum shoots as explants were inoculated in inducing bud differentiation medium (MS + 0.5mg / L 6-BA) were cultured , about 23d, vigorous plant growth , then explants inoculated on callus induction medium (MS + 0.2mg / L NAA + 2mg / L 6-BA) conducted subculture , the last part of shoots inoculated into rooting medium (1 / 2MS + 0.2mg / L NAA) cultured , 15d root, root number average length of 6.63 , the average root length 3.96cm, 21d after transplanting , the survival rate was 87.5% after transplanting . Following another part of explants cultured conduct Virus Free , then shoot tips cultured in medium (MS + 0.2mg / L 6-BA) , and after one week , beginning to turn green shoot tips ; 13d after start appear callus ; 28d after callus larger , some start sprouting , chrysanthemum shoot tip browning after three months , but did not take steps to cause death. It was found that the medium MS + 0.1mg / L NAA + 2mg / L 6-BA is suitable for buds proliferation , proliferation rate of up to 4.9 times the average ; medium MS + 1 mg / L NAA + 0.1mg / L 6-BA Chrysanthemum more conducive to induce root , root number per bottle average length 5.35 ; medium MS + 0.1mg / L NAA + 1mg / L 6-BA callus differentiation as well as the most suitable Chrysanthemum buds , bud induction rate 50 %.

毕业论文关键词：杭白菊；快速繁殖；茎尖脱毒

Keyword: Chrysanthemum morifolium Ramat; rapid propagation ; Virus Free

1 引言 5

1.1 杭白菊的介绍及现状 5

1.2 快速繁殖和茎尖脱毒的特点 5

1.3 快速繁殖技术技术介绍 6

1.4 实验研究方法 7

2 实验材料与方法 7

2.1 研究材料 7

2.1.1 植株与材料 7

2.1.2 培养基的配置 7

2.2 主要实验仪器和试剂 8

2.3 主要实验方法 9

2.3.1 材料消毒 9

2.3.2 接种 9

2.3.3 继代培养 9

2.3.4 茎尖脱毒 9

3 结果与分析 10

3.1 初代培养 10

3.2 继代培养 10

3.3 不同浓度激素对杭白菊根诱导的影响 10

3.4 不同浓度激素对丛生芽增殖的影响杭白菊的快速繁殖及茎尖脱毒培养:http://www.751com.cn/shengwu/lunwen_45432.html