低体温下小鼠的生理和代谢特性研究

摘要深度低体温技术在临床手术中的应用一直受到人们的关注，而寻找一种安全长时间的诱导低体温的手段成为很多研究人员的目标。在本实验中，我们对处于由5’-AMP诱导的深度低体温状态8h的小鼠的生理生化指标进行了检测，检测结果表明5’-AMP诱导的低体温对于小鼠的主要生理生化指标没有产生影响。随后我们对深度低体温状态对GalN/LPS诱导的肝损伤模型是否有保护作用进行了验证，结果显示低体温状态对于肝损伤的爆发起到了延缓作用。最后我们又成功将小鼠处于低体温状态的时间延长至16h。这一系列的结果表明使用5’-AMP诱导进入深度低体温状态的技术是一项安全且长时间的诱导低体温技术，有着很大的发展前景，对于深度低体温技术在更大范围的应用有很大的实际意义。63944

毕业论文关键词 5’-AMP 深度低体温延长时间

毕业设计说明书（论文）外文摘要

Title Physiological and metabolic properties of mice in deep hypothermia

Abstract The use of deep hypothermia in clinical surgery is attractive to many researchers, and looking for a safe and long time method for hypothermia become the goal of many researchers. In this study, we tested the biochemical indicators of the mouse after 8hours’ 5'-AMP-induced deep hypothermia. The result showed that 5'-AMP-induced deep hypothermia in mice did not affect the biochemical indicators of the mice. Then we validated if the deep hypothermia state can protect GalN/LPS-induced liver injury in mice. The result showed that the deep hypothermia delayed the outbreak of liver injury. We also successfully extended the deep hypothermia state to 16 hours. All the results revealed that the 5’-AMP induced deep hypothermia is a safe and prolonged hypothermia technique, suggesting that it has great prospects for further development. The 5’-AMP induced deep hypothermia technology has a great range of applications and significance.

Keywords 5’-AMP deep hypothermia extension

1 绪论 1

1.1 深度低体温 1

1.2 5’-AMP诱导小鼠深度低体温 1

1.2.1 5’-单磷酸腺苷（5’-AMP） 1

1.2.2 5’-单磷酸腺苷（5’-AMP）诱导深度低体温 2

1.3 GalN-LPS诱导的小鼠肝损伤模型 2

1.3.1 D-氨基半乳糖（ D-GalN） 2

1.3.2 LPS（lipopolysaccharide） 3

1.3.3 GalN-LPS肝损伤模型 3

1.4 肾上腺素（adrenaline,epinephrine,AD） 4

1.5 实验目的和意义 4

2 5’-AMP诱导的低体温与小鼠的生理代谢功能 5

2.1 材料和方法 5

2.1.1实验材料 5

2.1.2实验方法 5

2.1.3 统计方法 6

2.4 实验结果与讨论低体温下小鼠的生理和代谢特性研究:http://www.751com.cn/shengwu/lunwen_70816.html