聚季铵盐PDM对金葡菌的杀生作用与机理分析初探

摘要本文研究了聚二甲基二烯丙基氯化铵（PDM）对金黄色葡萄球菌的杀菌性能。以金黄色葡萄球菌为受试物种，配制中等污染程度的模拟水样，进行杀菌实验；采用平皿计数法，以杀菌率为考核指标，研究了PDM系列样品的作用浓度、作用时间和特征黏度（[η]值）对杀菌性能的影响；采用紫外吸收法，分别检测了PDM作用后菌悬液的蛋白质质量浓度与核酸吸光度的变化，以考察胞内物质泄漏情况；通过SEM观察了PDM作用前后细胞表面形态的变化。结果表明：针对菌含量为105个/mL的模拟水样，PDM([η]: 0.59～3.77)的作用浓度为0.05mg/L时，其24h杀菌率为22.98%～40.91%；作用浓度为0.5mg/L时，其24h杀菌率为98.92%～99.87%。PDM的作用浓度和作用时间对杀菌率的影响规律与特征黏度有关，特征黏度越大，所达到的最大杀菌率越大。PDM可以破坏菌细胞的壁、膜结构，使胞内物质泄漏，最终导致细胞死亡。因此，PDM系列样品对以金黄色葡萄球菌为代表的革兰氏阳性菌具有很好的杀灭效果，且PDM特征黏度越大，杀菌性能越强。64751

毕业论文关键词聚二甲基二烯丙基氯化铵（PDM）金黄色葡萄球菌杀菌性能杀菌机理

毕业设计说明书（论文）外文摘要

Title Preliminary Study on Bactericidal Action and Mechanism of Polyquaternary Ammonium Salt PDM against Staphylococcus aureus

Abstract

In this paper, the bactericidal performance of polydiallyldimethylammonium chloride (PDM) was researched. The bactericidal experiment was carried on by using Staphylococcus aureus as the tested species and preparing the simulated water samples with moderate degree of pollution. Using the bactericidal rate as assessment indicator, the effects of the action concentration, action time and intrinsic viscosity ([η] value) of PDM on the bactericidal performance were researched by plate counting method. The changes of protein concentration and nucleic acid absorbance after PDM acting bacterial suspension were detected by the UV absorption method to investigate the leakage of intracellular substances. The morphological changes of surface before and after PDM acting the cells were observed by SEM. The results showed that the 24h bactericidal rates of PDM([η]: 0.59~3.77) were 22.98%~40.91% when the of action concentration were 0.05mg/L, and that of PDM([η]: 0.59~3.77) were 98.92%~99.87% when the of action concentration were 0.5mg/L for the simulated water sample with bacteria content of 105cells/mL. The effects of action concentration and action time of PDM on the bactericidal rates were related to the intrinsic viscosity. The higher intrinsic viscosity value, the greater maximal bactericidal rate. PDM could damage the structures of cell wall and cell membrane to make the leakage of intracellular substances, and finally, the cell died. Therefore, serial PDM samples had an excellent bactericidal effect for Gram-positive bacteria represented by Staphylococcus aureus, and the higher intrinsic viscosity value, the better bactericidal performance.

Keywords Polydiallyldimethylammonium chloride (PDM) Staphylococcus aureus Bactericidal performance Bactericidal mechanism

1 引言 1

1.1 PDM简介 1

1.2 PDM的杀菌性能 1

1.2.1 PDM对革兰氏阴性菌的杀灭效果 2

1.2.2 PDM对革兰氏阳性菌的杀生效果 2

1.3 PDM的杀菌机理 2

1.4 本课题的研究内容和目标 3 聚季铵盐PDM对金葡菌的杀生作用与机理分析初探:http://www.751com.cn/shengwu/lunwen_72062.html