地表水体中多种抗生素分析方法的建立与验证

摘要：抗生素的滥用已经引起了重视。本文针对地表水体中抗生素污染问题，建立了地表水体中多种抗生素的HPLC-MS/MS分析方法和前处理方法，采用所建立的分析方法对太浦河中抗生素浓度含量进行研究。分析方法采用Waters XEvo TQ-S micro超高压液相色谱—质谱联用仪建立，前处理方法采用HLB固相萃取柱对目标物进行富集浓缩。该方法线性相关性均大于0.99、LOD均低于0.2 ng/L，回收率均大于80%，说明该方法能够很好的对地表水体中多种抗生素进行分析。采用该方法对太浦河流域抗生素取样分析结果表明，7种目标抗生素在地表水体中均有检出，其浓度均在ng/L水平，磺胺间甲氧嘧啶的平均浓度最高（47.17 ng/L），其次是磺胺氯哒嗪（19.99 ng/L）。47251

毕业论文关键词：抗生素地表水体超高压液相色谱—质谱联用仪太浦河

Establishment and validation of analytical method on 7 antibiotics in surface water

Abstract ：The abuse of antibiotics has been one of the hot topics. In this article, through the experiment established a variety of antibiotics in the surface water pretreatment method and instrument analysis method, and the concentration of antibiotics in TaiPu River content were analyzed by the analytical method. Instrument analysis method was performed on the Waters XEvo TQ-S micro HPLC-MS/MS system. And HLB cartridge was used in the pre-treatment method. The linear correlation were greater than 0.99, LOD method are less than 0.2 ng/L and the recovery were greater than 80%, shows that this method can very good analysis of many kinds of antibiotics in the surface water. Then the surface water in TaiPu River watershed were sampled and the concentrations of the antibiotics were analyzen by the method. The results showed that all 7 antibiotics were detected in the surface water, and all the concentrations were in ng/L levels, sulfanilamide between a oxygen pyrimidine has the highest average concentration (47.17 ng/L), followed by chlorine pyridazine sulfanilamide (19.99 ng/L).

Keywords： Antibiotic The Surface Water UHPLC MS/MS TaiPu River

1 绪论 1

1.1背景 1

1.1.1 抗生素简介 1

1.1.2 常见抗生素分类与危害 1

1.1.3 常用医药用抗生素进入地表水的途径与对生物可能造成的危害 2

1.1.4 抗生素的污染现状 2

1.2 目前地表水体中抗生素常见的分析方法 4

1.2.1 酶联免疫分析方法 4

1.2.2 气相色谱-质谱分析方法 4

1.2.3 毛细管电泳检测分析方法 4

1.2.4 高效液相色谱分析方法 5

1.2.5 超高液相-质谱联用分析方法 5

1.3 水样的前处理方法 5

1.3.1 固相萃取方法 6

1.3.2 液相微萃取方法 6

2 实验方法 7

2.1 实验仪器与试剂 7

2.2.1 实验试剂 7

2.2.2 实验仪器 7

2.2 磺胺类抗生素仪器分析方法的建立 8

2.2.1 抗生素溶液的配制 8

2.2.2 质谱方法的建立地表水体中多种抗生素分析方法的建立与验证:http://www.751com.cn/huaxue/lunwen_49401.html