摘要：生物能源作为化石燃料的理想替代能源，其清洁且可再生。但是细胞壁复杂的内部交联结构，使得木质纤维素构成了天然的抗降解结构，所以细胞壁结构特点的研究及其与降解转化的关系是很必要的。

本研究选取芒草和水稻为材料，分析其细胞壁主要成分，纤维素、半纤维素、木质素含量变化较大，芒草平均值：27.53±1.27,20.53±1.55，25.48±2.28（%干重），水稻平均值25.35±1.54,13.62±1.25,15.06±1.43（%干重），水稻可溶性糖含量较高；高温预处理可以提高纤维素降解转化效率，芒草不同材料之间总糖产率差异较大，五碳糖和六碳糖产率接近，体现出细胞壁结构的多样性，水稻不同材料之间差异较小，水解液以六碳糖为主；高温处理后，水稻的酶解产糖率高于芒草。53698

毕业论文关键词：芒草，水稻，高温预处理，酶解效率

Abstract: Biofuels as an ideal replacement of fossil fuel energy, its clean and renewable. But the cell wall complex internal crosslinking structure, make lignocellulose constitutes a natural resistance to degradation of the structure, so the cell wall structure research and its relationship with degradation of transformation is very necessary.

       This study selected miscanthus and rice as the materials, main component analysis of the cell walls, cellulose, hemicellulose, and lignin content change is bigger, miscanthus average: 27.53± 1.27, 20.53 ±1.55, 25.48 ± 2.28 dry weight (%), rice (average 25.35 ±1.54, 13.62 ±1.25, 15.06 ±1.43 dry weight (%), rice soluble sugar content is higher; High temperature pretreatment can improve the efficiency of transformation of cellulose degradation, miscanthus total sugar yield differences between different materials, five six carbon carbon sugar and sugar yield close to reflect the persity of the cell wall structure, rice little differences between different materials, the hydrolysate is given priority to with six carbon sugar; enzymatic hydrolysis of rice sugar rate is higher than miscanthus，After high temperature treat rice .

Keywords: Miscanthus，Rice， High temperature-pretreatment，Enzymatic hydrolysis

目   录

1 文献综述 1

1.1 生物质能 1

1.1.1 生物质概述 1

1.1.2 生物质能发展现状 1

1.2 植物细胞壁的结构 2

1.2.1 纤维素 3

1.2.2 半纤维素 3

1.2.3 木质素 4

1.2.4 果胶 4

1.2.5 细胞壁主要成分的连接方式 4

1.3 生物质降解转化 5

1.3.1 生物质降解转化流程 5

1.3.2 生物质预处理方式 5

1.4 本研究目的意义 7

2 材料与方法 7

2.1 实验材料 7

2.2 实验方法 8

3 结果与分析 12

3.1 细胞壁成分测定 12

3.1.1 六碳糖、五碳糖标准曲线 12

3.1.2纤维素、半纤维素、木质素测定 13

3.2 高温预处理降解转化效率 14