摘 要：采用铅镉胁迫、UV-B辐射胁迫及复合胁迫分别处理蚕豆种子及幼苗，研究3种胁迫处理下蚕豆种子的萌发情况及蚕豆幼苗根系和叶片生理指标的变化。结果显示，3种胁迫处理下，随着胁迫处理天数的延长，蚕豆种子的发芽率、蚕豆幼苗根系生长速度、根系活力、蚕豆幼苗叶片内叶绿素含量、可溶性蛋白含量均呈降低趋势；而蚕豆幼苗叶片内丙二醛含量和过氧化物酶含量呈升高趋势。各项指标对3种胁迫的敏感程度为：复合胁迫＞UV-B辐射胁迫＞铅镉胁迫。上述分析说明3种胁迫处理对蚕豆种子萌发、蚕豆幼苗生长及蚕豆幼苗根系和叶片生理指标均有不同程度的影响。
关键词：蚕豆；铅镉胁迫；UV-B辐射；生理特性；根系活力
Response of Physiological Indexes of Root System and Leaves from
Broad Beans Seedling toward UV-B Radiation and Pb and Cd Stress
Abstract: Broad bean seeds and seedlings were adopted to study the changes of seed germination and physiological indexes of root system and seedling leaves under pb and Cd stress, UV-B radiation stress and compound stress. The results were following: with the extension of stress treatment days, the germination rate of seed, the growth rate of seedling root, root vigor, chlorophyll content and soluble protein content of seedling leaves showed a decreased tendency; MDA content and peroxidase content of seedling leaves showed rising trend instead. The level of sensitivity of each index toward three kinds of stress was following: compound stress was greater than UV-B radiation stress and UV-B radiation stress was greater than pb and Cd stress.The above statement showed that seed germination and seedling growth and physiological indexes of root system and seedling leaves of broad beans were subject to the impact of varying degrees under three kinds of stress treatments.
Key words: Broad beans; pb and Cd stress; UV-B radiation; Root vigor; Physiological characteristics
目 录
摘 要 1
引言 2
1. 材料与方法 2
1.1实验材料 2
1.2 实验方法 2
1.2.1 铅镉胁迫液的制备 2
1.2.2 种子处理 2
1.2.3 实验处理设计 3
1.2.4 项目测定 3
2. 结果与分析 3
2.1 铅镉溶液、UV-B辐射、复合胁迫对蚕豆种子萌发的影响 3
2.2 铅镉溶液、UV-B辐射、复合胁迫对蚕豆根系的影响 4
2.3 铅镉溶液、UV-B辐射、复合胁迫对蚕豆幼苗叶绿素含量的影响 5
2.4 铅镉溶液、UV-B辐射、复合胁迫对蚕豆幼苗可溶性蛋白含量的影响 5
2.5 铅镉溶液、UV-B辐射、复合胁迫对蚕豆幼苗丙二醛含量的影响 6
2.6 铅镉溶液、UV-B辐射、复合胁迫对蚕豆幼苗过氧化物酶活性的影响 6
3. 结论与讨论 7
3.1 结论 7
3.2 讨论 7
参考文献 8
致谢 10，3636