摘要： β-苯乙醇是具有玫瑰花香的重要香料化合物，采用微生物转化生成天然β-苯乙醇是目前极具工业化价值的途径之一。本课题通过从茶树、桂花树、玫瑰花土壤样品以及酒糟样品和玫瑰花酵素样品中采集菌种，采用菌种筛选分离的方法对转化生成β-苯乙醇的微生物菌株进行筛选，并采用气相色谱分析、GC-MS分析、聚合酶链式反应等生物学手段对获取菌种进行鉴定，最终鉴定出玫瑰花土壤样品（C）、酒糟样品（E）筛选出了产β-苯乙醇的菌株，分别为菌C-2、菌E-2，桂花树（A）茶树（B）土壤样品中筛选出了产出苯乙醛的菌株。47059
毕业论文关键词： β-苯乙醇；生物转化；L-苯丙氨酸
Screening and identification of strains of β-phenylethanol production and preliminary study on conversion process
Abstract: β-phenethyl alcohol is an important Fragrance compounds having a rose scent, the use of microbial conversion generate natural β-phenethyl alcohol is one way to present highly industrialized value. This topic by collecting bacteria from tea, sweet-scented osmanthus trees, roses and soil samples and distiller's grains samples and roses enzyme samples, the method of screening of strains isolated on the conversion generates β-phenethyl l alcohol screening microbial strains, and the use of gas chromatography analysis, GC-MS analysis of biological means of polymerase chain reaction to get the bacteria were identified, and ultimately identified soil samples roses (C), distiller's grains sample (E) screened the strains producing β-phenethyl alcohol, bacteria were C-2, strain E-2, osmanthus tree (a) tea tree (B) soil samples were screened out strains output phenylacetaldehyde.
KeyWords: β-phenylethanol；Bioconversion；L-Phenylalanine
目 录
1 绪论 1
1.1 β-苯乙醇产品概况 1
1.1.1 β-苯乙醇的理化性质 1
1.1.2 β-苯乙醇的用途 1
1.1.3 β-苯乙醇的合成方法 2
1.2 合成β-苯乙醇的微生物 2
1.3 微生物转化法合成β-苯乙醇的代谢途径 3
1.4.1 国内研究现状 4
1.4.2 国外研究现状 5
1.5 选题背景及意义 6
2 实验部分 6
2.1 实验材料与仪器 6
2.2 培养基配制 7
2.2.1 培养基配置原则 7
2.2.2 产β-苯乙醇菌种筛选的培养基 7
2.2.3 产β-苯乙醇菌种的转化培养基 9
2.3 实验方法 9
2.3.1 菌种的初步筛选 9
2.3.2 菌种的生物转化实验 10
2.3.3 产物分析和菌种鉴定 10
3、结果与讨论 13
3.1 筛菌结果 13
3.2 生物转化实验 14
3.2.1 气相分析 14
3.2.2 GC-MS分析 16
3.3 PCR与凝胶电泳 产β-苯乙醇菌株的筛选鉴定及其转化工艺初步研究:http://www.751com.cn/shengwu/lunwen_48987.html