顶空固相微萃取法国内知名白酒挥发性成分的鉴定

摘要：选取国内5种知名品牌白酒，利用顶空固相微萃取法（HS-SPME）提取酒样中的挥发性成分，通过气相色谱—质谱联用(GC-MS)技术，分别对5种酒的挥发性成分进行分析，检测出白酒酒样中的挥发性物质。在该条件下5种酒分析出180种挥发性物质，其中酯类物质100种，醇类物质32种，醛类物质14种，酸类物质9种，酮类物质13种，酚类物质4种，烷烃类8种，呋喃类5种，烯烃类2种，含氮化合物2种，含硫化合物1种。研究发现白酒中除酯类、醇类、酸类、醛酮类，酚类、呋喃类等主要的挥发性物质外，还有少量含硫化合物，因此，借助气相色谱-火焰光度检测器（GC-FPD）对含硫化合物检测高效、专一性强的特点，建立了一种简单、快速检测白酒中含硫化合物的方法，利用该方法在5种白酒检测出22种含硫化合物。63210

毕业论文关键词：白酒；顶空固相微萃取（HS-SPME）；气相色谱-质谱（GC-MS）；挥发性成分；气相色谱-火焰光度检测器（GC-FPD）

Characterization of Volatile Compounds of Chinese Famous Liquors by HS-SPME-GC-MS and GC-FPD

Abstract：In order to research volatile compounds in liquors, 5 Chinese famous liquors had been selected and investigated. The volatile compounds in liquors were extracted by headspace solid-phase microextraction (HS-SPME).And then the volatile compounds were analyzed by gas chromatography-mass spectrometry（GC-MS）. A total of 180 volatile compounds were detected and identified , including 100 esters, 32 alcohols, 14 aldehydes, 9 acids, 13 ketones and 4 phenols, 8 acetals, 5 furfurans, 2 alkenes, 2 nitrogenous compounds, and 1 sulfuric compound. Several sulfur compounds were found in liquors in addition to esters, alcohols, acids, aldehydes and ketones, phenols, furfurans and so on. Therefore, A simple and rapid method to detect sulfur compounds in liquors was established with the help of high efficiency and specificity of gas chromatography-flame photometric detector(GC-FPD) to sulfur compounds. Moreover, a total of 22 sulfur compounds in five liquors were detected.

KeyWords: Chinese liquors; HS-SPME; GC-MS; volatile compounds; GC-FPD

1 绪论 1

1.1 白酒概述 1

1.2 白酒的研究现状 2

1.3 白酒挥发性成分的分析方法 4

1.3.1 顶空固相微萃取（HS-SPME）技术 4

1.3.2 气相色谱-质谱连用（GC-MS）技术 5

1.3.3 气相色谱-火焰光度检测器（GC-FPD）检测技术 6

1.4 本课题的研究目的和意义 7

2 实验部分 8

2.1 原料、试剂和仪器设备 8

2.1.1 实验原料 8

2.1.2 实验试剂 8

2.1.3 实验仪器设备 8

2.2 样品处理方法和分析条件 8

2.2.1 顶空固相微萃取（HS-SPME） 8

2.2.2 气相色谱-质谱连用（GC-MS） 9

2.2.3 气相色谱-火焰光度检测器（GC-FPD） 9

2.3挥发性化合物定性分析 9

2.4挥发性化合物的定量方法顶空固相微萃取法国内知名白酒挥发性成分的鉴定:http://www.751com.cn/shiping/lunwen_69619.html