食品级壳寡糖对甘薯黑斑病菌抑制作用的初步研究

摘要：为研究壳寡糖对甘薯黑斑病菌的抑制作用，设置了几个实验条件：不同种类壳寡糖、同种壳寡糖浓度不同、pH不同。结果表明：甘薯黑斑病菌在含有4种不同种类壳寡糖的PDA培养基上均能生长，其中在含有编号327的壳寡糖培养基上菌落直径最大，在含有编号824的壳寡糖培养基上菌落直径最小；分生孢子产孢量以含有编号327的壳寡糖培养基最多，含有编号824的壳寡糖培养基最少。表明编号824的壳寡糖对甘薯黑斑病菌的抑制作用最强。当pH值为5时，编号824的壳寡糖对甘薯黑斑病菌抑制作用最强，菌落直径最小，分生孢子产孢量最低。本实验为甘薯产业中采用壳寡糖预防和治疗甘薯黑斑病奠定了理论基础。69044

毕业论文关键词：甘薯黑斑病菌；壳寡糖；抑菌作用；培养条件

The Preliminary study of Several Food Grade Oligochitosan to the Ceratocystis fimbriata on the inhibitory effect

Abstract: The inhibitory effect of Oligochitosan on Ceratocystis fimbriata was tested with different kinds of Oligochitosan, Oligochitosan concentration and pH value. The results indicated that the pathogen could grow on PDA culture media with four different kinds of Oligochitosan, the maximum bacterial colony diameter was observed on the culture media with Oligochitosan 327, while the minimum bacterial colony diameter was observed on the culture media with Oligochitosan 824. The highest amount of spore production was obtained in the culture media with Oligochitosan 327, while least one appeared in the culture media with Oligochitosan 824. This suggested that the Oligochitosan 824 had the strongest inhibitory action on Ceratocystis fimbriata. When the pH value was 5, the Oligochitosan 824 showed the strongest inhibitory action on Ceratocystis fimbriata, with the lowest colony diameter minimum and conidial sporulation. This experiment laid a theoretical foundation for sweet potato industry using Oligochitosan for prevention and treatment of Ceratocystis fimbriata

Key words: Ceratocystis fimbriata; Oligochitosan; Inhibitory effect; Culture conditions

引言 1

1 实验材料与方法 2

1.1 实验材料 2

1.2 实验方法 2

2 实验结果与分析 4

2.1 四种壳寡糖样品在0.5 mg/mL浓度下对甘薯黑斑病菌的抑制作用 4

2.2 四种壳寡糖样品在1 mg/mL浓度下对甘薯黑斑病菌的抑制作用 6

2.3 四种壳寡糖样品在2 mg/mL浓度下对甘薯黑斑病菌的抑制作用 7

2.4 不同pH值下壳寡糖对甘薯黑斑病菌的抑制作用 8

3 讨论 10

致谢词 11

参考文献 11

引言

甘薯是我们日常饮食中不可缺少的一种食物，世界甘薯种植面积最多的是亚洲，中国是亚洲各国中甘薯产量最多的国家，每年的甘薯种植面积约占全国耕地面积的4.2％，中国甘薯种植面积约占世界60％，产量约占世界总产的80％。甘薯产业非常发达，已广泛应用于食品、医疗、造纸、化工及新能源等十多个工业门类，以甘薯为原料的产品高达400多种。甘薯黑斑病又称黑疤病，是真菌病害，由甘薯长喙壳菌侵染所致 [1]，全球甘薯产区均有发生此病害。十九世纪九十年代，美国首先发现了甘薯黑斑病，29年后传入日本，1937年由日本鹿儿岛传入我国辽宁省盖县[2]。接着，该病在我国甘薯产区迅速蔓延，造成了广泛而严峻的危害。该病使得我国的甘薯产量每年亏损5％~10％，危害严重时为20％~50％[3]乃至更高。另外，人和家畜若误食病薯会中毒，因为其中含有毒素黑疱霉酮。工业上的发酵常用薯块等作为发酵原料，若使用病变的薯块会造成酒精的产量和质量下降，原因是发酵过程中重要的的酵母菌和糖化酶菌被毒害[4]。论文网食品级壳寡糖对甘薯黑斑病菌抑制作用的初步研究:http://www.751com.cn/shiping/lunwen_77784.html