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摘要：以芘为荧光探针，二苯甲酮为猝灭剂，采用稳态荧光探针法测定了聚氧乙烯（21）硬脂基醚（Brij721）表面活性剂增溶芘后的稳态荧光光谱，建立了Brij721非离子表面活性剂的临界胶束浓度（CMC）和临界胶束聚集数（Nm）的测试方法。根据I322/I337与Brij721浓度变化的关系，可得到Brij721的临界胶束浓度为3.45×10-6mol/L。确定荧光探针芘的适宜浓度Cp为6×10-7mol/L，猝灭剂的适宜浓度CQ范围取为0.002～0.003mmol/L，表面活性剂溶液浓度为3~12倍CMC时，胶束聚集数(N)随表面活性剂浓度增大而线性增大，并用线性拟合作图法得到Brij721的临界胶束聚集数为9。同时，外部添加的试剂如无机盐、乙醇均影响其临界胶束浓度。7833
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关键字：荧光探针法；临界胶束浓度；临界胶束聚集数；脂肪醇聚氧乙烯醚
Determination of Critical Micelle Concentration (CMC) of Nonionic Surfactant of Fatty Alcohol-polyoxyethylene Ether by Steady-State Fluorescence Probe Method
Abstract: Taken the pyrene as the fluorescence probe and diphenyl ketone as quencher, the steady-state fluorescence spectrum of Polyoxyethylene (21) stearyl ether (Brij721) surfactant was determined by fluorescence probe method. A sensitive method for determining critical micelle concentration (CMC) and critical micelle aggregation number (Nm) of Brij721 surfactant was established. According to the change of fluorescence intensity ratio of pyrene I322/ I337 with the change of surfactant concentration of aqueous solution, CMC value for Brij721 was 3.45×10-6 mol/L. Ensure the proper concentration of fluorescence probe is 6×10-7mol/L, the proper concentration range of quencher is 0.002~0.003mmol/L, the aggregation number (N) increases linearly with the increase of Brij721 concentration in the range of 3~12 times of CMC and Nm which is 9 can be extrapolated from N-C fitting curve. In addition, the externally added reagents such as salts and ethanol also influence CMC.
Key Words: fluorescence probe method; critical micelle concentration; micelle aggregation number; fatty alcohol-polyoxyethylene ether
目 录
1 前言 1
1.1 脂肪醇聚氧乙烯醚非离子表面活性剂研究现状 1
1.2 脂肪醇聚氧乙烯醚非离子表面活性剂的应用 1
1.3 表面活性剂的临界胶束浓度概念 1
1.3.1 表面活性剂胶束化作用的定义 2
1.3.2 临界胶束浓度的作用及意义 2
1.4 关于临界胶束浓度的测定方法 2
1.4.1 表面张力法 2
1.4.2 电导率法 3
1.4.3 染色法 3
1.4.4 浊度法 3
1.4.5 共振光散射法 3
1.4.6 超滤法 4
1.4.7 吸附伏安法 4
1.4.8 紫外分光光度法 4
1.4.9 微分吸附计时电位法 4
1.4.10 荧光探针法 5
1.5 影响临界胶束浓度的因素 5
1.6 胶束聚集数及其测定方法 6
1.7 本课题研究的目的和意义 6
2 实验部分 7
2.1 实验原料 7
2.2 实验仪器与设备 7
2.3 实验溶液的配制 7
2.3.1 测定CMC所需各标准溶液的配制 7
2.3.2 确定合适猝灭剂浓度所需各溶液的配制 10
2.3.3 测定临界胶束聚集数所需溶液的配制 11
2.4 测定方法 11
2.4.1 不同浓度Brij721溶液中芘的激发光谱测定 11
2.4.2 不同浓度Brij721溶液中芘的发射光谱测定 11