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摘要：细菌纤文素(Bacterial Cellulose, BC)作为一种新型的复合材料，在各个领域都已经被广泛使用。它有独特的性能，包括高吸水性、高保水性、高透过性等。本实验主要利用木醋杆菌，通过静态发酵法制备细菌纤文素/聚丙烯酰胺复合膜，并测定复合膜的物理和机械性能，包括含水量、复水率、拉伸强度、断裂伸长率以及红外观测，并研究不同浓度的聚丙烯酰胺浓度对BC复合膜的影响。结果表明聚丙烯酰胺浓度为0.1%时，含水量最高为98.50%；浓度为0.05%时，复水率最高为96.87%；浓度为0.4%时，拉伸强度，压缩强度最大，拉伸强度为0.3487Mpa，压缩强度为0.3616Mpa；浓度为0.2%时，断裂伸长率最高为21.98%。19501
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关键词： 木醋杆菌；细菌纤文素；聚丙烯酰胺；物理和机械性能；红外
Preparation and Characterization of Bacterial Cellulose / PAM Composite Membrane
Abstract: Bacterial Cellulose (Bacterial Cellulose, BC) as a new kind of composite material has been widely used in various fields. Its has unique properties, such as high water absorb ability, high water retention ability, high permeability, and so on have been many cognitive and accept it. This experiment makes use of Acetobacter xylinum to produced the bacterial cellulose/poly (vinyl alcohol) composite membrane in static conditions. Then determined the physical and mechanical properties of the membrane, including water holding capacity, recovery rate of water, tensile strength, elongation break ,infrared observations, and studied the influence of different concentrations of PAM on BC composite membranes. The results showed that when the polyacrylamide concentration was 0.1% ,the water content was the highest, when the polyacrylamide concentration was 0.05% ,the rate of rehydration was the highest namely 98.50%.When the polyacrylamide concentration was 0.4% , the compressive strength and the tensile strength were the highest, the compressive strength was 0.3487 Mpa and the tensile strength was 0.3616 Mpa. When the polyacrylamide concentration was 0.2% , the elongation at break was the highest namely 21.98%.
Key words: Acetobacter xylinum; bacterial cellulose; PAM; physical and mechanical properties; infrared spectroscopy
目录
1绪论 1
1.1 细菌纤文素简介 1
1.1.1 细菌纤文素的结构与性质 1
1.1.2 细菌纤文素的合成过程及生成的基本条件及其发酵生产 1
1.1.3 细菌纤文素的应用 2
1.2 聚丙烯酰胺简介 4
1.2.1 聚丙烯酰胺的理化性质 4
1.2.2 聚丙烯酰胺的应用 4
1.3 细菌纤文素的改性及其复合材料 5
1.4本课题的研究的内容和目的 6
1.4.1 本课题研究的内容 6
1.4.2 本课题研究的目的 6
1.5本课题的研究的意义 6
2 实验物品与方法 7
2.1实验物品 7
2.1.1 实验试剂及原料 7
2.1.2 实验主要仪器 7
2.1.3 实验菌种 8
2.1.4 培养基 8
2.1.5 实验主要溶液 8
2.2实验方法 9
2.2.1 pH测定 9
2.2.2 含水量的测定 9
2.2.3 复水率的测定 9
2.2.4 红外光谱测试 9
2.2.5 物理性质的测定 9
2.3发酵方法 10
2.3.1 溶液配制 10
2.3.2 木醋杆菌种子液的制备 10
2.3.3 细菌纤文素膜的制备 10
2.3.4 细菌纤文素膜的提取和处理 10