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摘要：棉花是我国主要经济作物，化学脱叶是棉花机械化收获过程中一项重要技术措施。化学脱叶技术是运用植物生长调节剂（噻苯隆），干预棉花生理生化过程，加速植株衰老脱叶，从而达到提高机采棉花品质的目的。为了探明噻苯隆对棉花叶片脱落的效应，本研究在江苏省农科院用噻苯隆处理棉花叶片，同时使用H2O2抑制剂DPI后喷施噻苯隆，于不同药效天数测定脱落率、相对含水量、叶片H2O2含量以及叶柄离层的H2O2分布状况。结果表明：0.1%噻苯隆浓度处理下的叶片脱落率和H202含量较对照组几乎同时升高，叶柄离层的H2O2分布明显，而加入DPI后喷施噻苯隆的脱叶率显著降低。本研究结果证明H2O2参与了噻苯隆对棉叶脱落的调控，为噻苯隆更深层次的分子调控机制研究奠定了基础。25544
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毕业论文关键词：棉花；脱叶；噻苯隆；H2O2
The Effect of Thidiazuron on Defoliation in Cotton
Abstract： Cotton is a major economic crop in our country. With the development of mechanization technology applied in the process of cotton harvest, chemical defoliation plays an important role in the process, which helps save a lot of costs and energy. The key of chemical defoliation technology is making use of plant growth regulator such as thidiazuron, in order to intervene physiological and biochemical processes in cotton, then accelerate the speed of senescence and defoliation and achieve the purpose of improving the yield and quality of cotton. In order to elucidate the effect of thidiazuron on defoliation in cotton,our study made a treatment under the optimum concentration of thidiazuron as well as DPI at the Jiangsu Provincial Academy of Agricultural Sciences, and then we measured the defoliation rate, relative water content, the content of H2O2 and distribution of H2O2 in petiole at different days. The results showed that the defoliation rate of leaves and the content of H2O2 under the 0.1% thidiazuron concentration were almost increased compared with the control group and the H2O2 distribution of the petiole was obvious.At the meantime, the defoliation rate of leaves was decreased than that of the control group after adding H2O2 inhibitor DPI . Therefore, we clarify the H2O2 involved in thidiazuron regulated the signal pathway of cotton leaves.
Key words: Cotton；Defoliation；Thidiazuron；H2O2
目 录
摘要3
关键词3
Abstract3
Key words3
1 引言（或绪论）3-5
1.1 噻苯隆对棉花化学脱叶的作用3-4
1.2 叶片衰老脱落的原理研究进展4
1.2.1 国内关于叶片衰老脱落原理研究进展4-5
1.2.2 国外关于叶片衰老脱落原理研究进展4
2 材料与方法5-6
2.1 试剂和仪器5-6
2.2 试验方法6
2.3 测定内容与方法6
3 结果与分析6-9
3.1 不同浓度TDZ对棉花叶片脱落率（%）与相对含水量（%）的影响6-8
3.2 0.1%TDZ对棉花叶片脱落率的影响6-7
3.3 0.1%TDZ对棉花叶片H2O2含量的影响7
3.4 0.1%TDZ对棉花叶柄离层H2O2含量的影响8
3.5 TDZ处理下H2O2抑制剂对棉花叶片脱落的影响 8-9
4 讨论9-10
4.1 不同噻苯隆浓度对棉花脱叶的影响9
4.2 H2O2参与噻苯隆调控棉花叶片脱落的直接证明9-10
4.3 H2O2参与噻苯隆调控棉花叶片脱落的间接证明10
致谢10-11
参考文献11-12
噻苯隆对棉花叶片脱落的效应研究
1 引言
1. 1 噻苯隆对棉花化学脱叶的作用
棉花是我国主要经济作物，化学脱叶是棉花机械化收获过程中必不可少的一项重要技术措施。化学脱叶技术是人工使用化学脱叶剂（噻苯隆），干预作物的生理生化过程，使其提前衰老脱叶的一种技术，从而有利于作物的机械化收获。噻苯隆在生产中具有优良的脱叶效果，噻苯隆的适量使用，能够合理调控棉花叶片脱落，从而降低生产成本并且有效提高机采棉花产量和品质。因此，加强对噻苯隆的脱叶机理的研究，将对其在生产中的合理施用技术提供重要理论依据。