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摘 要 ：SUMO( small ubiquitin — related modifier）化修饰是细胞内蛋白质功能调节的重要方式之一,它是广泛存在于真核生物中高度保守的蛋白家族 ，在脊椎动物中有三个SUMO基因。SIZ1 在植物的抗寒性 、抗热性 、抗旱性 、磷酸饥饿反应 、抗病防御以及花期调控等生理过程中都发挥着重要作用。文章主要运用生物信息学的方法，利用拟南芥AtSIZ1基因在陆地棉中进行了电子克隆并没有检索到明确结果。利用拟南芥氨基酸或核苷酸序列在雷蒙德氏棉、亚洲棉数据库中比对检索获得序列，两者是串联顺向重复。利用雷蒙德氏棉、亚洲棉siz1a/b氨基酸或核苷酸序列在陆地棉ESTs数据库中比对检索获得序列9条序列，四个序列的比对结果完全一样。利用CAP3 Sequence Assembly Program，对陆地棉5’端和3’端ESTs分别进行组装获得了GhSIZ1基因5’端和3’端ESTs核酸序列。利用生物信息学的方法进一步对其DNA碱基序列与蛋白质氨基酸序列进行了分析，旨在为更好地研究基因SIZ1提供一定的理论资料。28531
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毕业论文关键词： SIZ1；SUMO；陆地棉; 电子克隆
In Silico Cloning and Analysis of GhSIZ1 Gene
Abstract：SUMO (small ubiquitin-related modifier) modification is one of the important ways of intracellular protein function adjustment, it is widely exists in eukaryotic highly conserved protein family, there are three type gene in vertebrates. SIZ1 in cold resistance, heat resistance, drought resistance, phosphate starvation response, antiviral defense, flowering regulation and physiological processes plays an important role in plant. This paper mainly using bioinformatics methods, the use of Arabidopsis thaliana AtSIZ1 gene to the upland cotton in electronic cloning is not retrieved clear results. Using Arabidopsis amino acid or nucleotide sequences in Raymond's cotton, cotton in Asia than in the database retrieval sequence, the two are in series consequent repetition. Use Raymond's cotton, cotton siz1a Asia/b amino acid or nucleotide sequences in upland cotton ESTs database than the retrieval for nine sequences, four sequence alignment results are exactly the same. Use CAP3 Sequence Assembly Program of upland cotton 5 'end and 3' end ESTs were assembled obtained GhSIZ1 gene 5 'end and 3' end ESTs nucleic acid Sequence. Using bioinformatics methods to further its DNA base sequence and protein amino acid sequence was analyzed, and aims at providing a better study SIZ1 gene with certain theoretical data.
Key Words: SIZ1; SUMO; Gossypium hirsutum; Cloning in silico
目 录
摘 要 1
引言 2
1陆地棉SIZ1电子克隆方法与步骤 6
1.1获得Atsiz1的基因和蛋白序列 6
1.2直接在陆地棉ESTs库中blast获得EST片段 6
1.3获得雷蒙德氏棉、亚洲棉SIZ1基因序列 6
1.4获得陆地棉EST片段 6
1.5获得陆地棉GhSIZ1基因核苷酸序列 6
2陆地棉SIZ1电子克隆的结果与分析 7
2.1Atsiz1的基因和蛋白序列 7
2.1.1 Atsiz1基因核苷酸序列 7
2.1.2 Atsiz1基因的蛋白质序列 7
2.2雷蒙德氏棉、亚洲棉数据库中比对获得检索序列 8
2.2.1 亚洲棉Gasiz1a核苷酸序列 8
2.2.2亚洲棉Gasiz1a氨基酸序列 8
2.2.3亚洲棉Gasiz1b核苷酸序列 9
2.2.4亚洲棉 Gasiz1b氨基酸序列 9
2.2.5雷蒙德氏棉Grsiz1a基因核苷酸序列 10
2.2.6雷蒙德氏棉Grsiz1a基因氨基酸序列 10
2.2.7雷蒙德氏棉Grsiz1b基因核苷酸序列 10