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摘要1, 2, 4-三氮唑广泛应用于医药、农药、化工等多行业，是一种典型的难降解含氮杂环化合物。本文对前期工作筛选出的一株新型三氮唑降解菌NJUST26进行表征与鉴定，研究其生物降解三氮唑的特性。实验结果表明，NJUST26菌为Shinella sp.，是第一株发现可以利用三氮唑作为其唯一的碳源、氮源和能源的微生物。在pH5.0-8.0的范围内，菌种可实现三氮唑的完全降解，且最佳降解条件为30ºC、pH 6-7。本文还探讨生长因子(碳源和氮源)对NJUST26菌降解三氮唑的影响。结果表明：(I) NH4+ (5mM、10mM、20mM) 和 NO3- (5mM、10mM)都能促进NJUST26菌降解三氮唑，但NO2- (5mM、10mM、20mM)和高浓度的NO3- (20mM)有明显的抑制作用。(II)低浓度条件下(0.5g/L)，酵母促进三氮唑降解的作用最为显著，其次是葡萄糖和蔗糖。然而高浓度条件下(2g/L)，酵母和蔗糖则抑制降解，葡糖糖仍加速三氮唑降解。后续的反应器实验表明添加NJUST26菌到反应器显著加强了其生物降解三氮唑的能力。30617
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关键词 三氮唑 特效菌 Shinella sp. 生物降解
毕业论文设计说明书外文摘要
Title Characterization of a new 1,2,4-trizole degrading strain and optimization of the degradation conditions
Abstract
1,2,4-triazole, which is a typical refractory nitrogen heterocyclic compounds, is widely used in medicine, pesticide, chemical and other industries. The identification and characterization of a new triazole degrading strain and its incubation conditions for degradation of triazole were investigated in this paper. The results showed that, the new strain, named NJUST26, which could utilize triazole as the sole carbon, nitrogen and energy source, was identified as Shinella sp.. The strain could completely degrade triazole in pH 5.0-8.0. The optimum temperature and initial pH for triazole degradation were 30℃ and pH 6-7, respectively. The effects of different nutrition condition including various nitrogen and carbon source on triazole biodegradation by NJUST26 were investigated. (I) NH4+ (5mM、10mM、20mM) and NO3- (5mM、10mM) facilitate triazole degradation, while NO2- (5mM、10mM、20mM) and high concentration of NO3- (20mM) provide inhibition effect. (II) Low concentration of yeast (0.5g/L) has the most remarkable effect on triazole degradation, followed by glucose and sucrose. But high concentration of yeast and sucrose will inhibit degradation, through glucose still can accelerate triazole degradation. In addition, the reactor experiment indicated that the dosing of NJUST26 to the reactor system could remarkably enhance the triazole biodegradation ability.
Keywords Triazole Specific bacteria Shinella sp. Biodegradation
目 次
1 引言 1
1.1 三氮唑的简介 1
1.2 三氮唑结构与性质 1
1.3 三氮唑的应用 2
1.4 三氮唑的危害 2
1.5 三氮唑废水的处理方法 2
1.6 课题研究的目标和意义 4
2 材料与方法 5
2.1 实验材料 5
2.2 实验方法 6
2.3 实验表征方法 8
3 结果与讨论 9
3.1 表征与鉴定 9
3.2 影响因素的确定 10
3.3 三氮唑废水生物强化处理 15
结论 17
致谢 18
参考文献 19
附录A Shinella sp. NJUST26的16SrDNA 序列表 22
1 引言
1.1 三氮唑简介
三氮唑是含氮杂环化合物的典型代表，是大量复杂含氮杂环化合物的基本结构单元，是所有细胞和生命物质的一个重要组成部分[1]。近年来，三氮唑因其特有的高生物活性和低毒性，成为医药和农药研究和开发的重要结构组成单元。