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摘要：粪菌移植(FMT)作为一种治疗手段，已在人类肠道疾病中有较多应用，但在新生仔猪上的研究尚为少数。本试验通过MiSeq高通量测序技术，研究早期母源粪菌干预对杜长大新生仔猪肠道菌群变化的影响。选取一窝14头仔猪，出生后，随机分为粪菌处理组和对照组。粪菌接种组仔猪出生后1-5天每天灌注粪菌接种液（接种量逐渐增大，前三天2 mL/天，后两天3 mL/天），对照组灌注等量的生理盐水。于1，3，5，7，10，14，18，22日龄采集仔猪粪样，并于仔猪出生后第19，20，21，23，26天采集母猪粪样。高通量测序结果显示，灌喂母源粪菌在一定程度上增加了仔猪的肠道微生物的多样性；两组仔猪肠道中的变形菌门的丰度均随日龄增加而显著降低，而厚壁菌门的丰度显著增加，且从10日龄起，拟杆菌门和厚壁菌门之和约为90%；同时灌喂母源粪菌也改变了如Holdemanella、uncultured Ruminococcaceae、Prevotellaceae NK3B31 group、Faecalibacterium和Ruminococcaceae UCG-010在内的某些菌属的丰度。同时发现在1-3日龄，口服灌喂母源粪菌液并不能影响仔猪肠道菌群的定植，这一阶段主要受母体微生物结构的影响；灌喂粪菌液对仔猪肠道菌群定植的影响最多持续10-14天；而且仔猪在22天左右，肠道菌群结构逐渐趋同于母猪肠道菌群。38700
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毕业论文关键词：粪菌移植；新生仔猪；肠道菌群
Effect of oral maternal fecal microbiota on intestinal flora development of newborn piglets
Abstract：As a treatment, fecal microbiota transplantation has been used in human intestinal disease,but the research on newborn piglets is still limited. This study was conducted by MiSeq high throughput sequencing technology to investigate the effects of oral maternal fecal intervention on intestinal flora in Duroc × Landrace × Large newborn piglets. We chose 14 piglets which had the same mother, were randomly pided into fecal treatment group and control group. The fecal inoculation group was fed with fecal inoculation solution on the 1-5 days after birth (the volume of the solution gradually increased, 2 mL/d in the first three days, 3 mL/d in the later two days). The control group was perfused with equivalent saline. Piglets’ fecal sample were collected at 1, 3, 5, 7, 10, 14, 18 and 22 days old, and sow’s fecal samples were collected at 19, 20, 21, 23 and 26 days after piglets’ birth. High throughput sequencing results are shown that oral administration of maternal fecal fluid could increase the persity of gut microbiota in piglets to some extent. The proportion of Proteobacteria was decreased significantly, while Bacteroidetes gradually became one of the dominant phyla. From 10 days after birth, the sum of the proportion of Firmicutes and Bacteria was about 90%. Meanwhile, the treatment also significantly changed the abundance of some genus, including Holdemanella, uncultured Ruminococcaceae, Prevotellaceae NK3B31 group, Faecalibacterium and Ruminococcaceae UCG-010. During the first 3 days after birth, Oral administration of maternal fecal fluid did not affect the colonization of intestinal microbiota in piglets, maternal bacteria was considered as the main factor in determining the gut bacterial structure. The effect of oral maternal fecal turbid liquid on intestinal flora’s colonization of piglets was for a maximum of 10 to 14 days. And the cluster of intestinal flora of piglets was close to the sows’ in about 22 days after birth.
Key words: fecal microbiota transplantation; newborn piglets; intestinal flora
目 录
摘要3
关键词3
Abstract3
Key words3
前言3
1材料与方法4
1.1试验材料 4
1.2试验方法 4
1.2.1母源粪菌的制备4
1.2.2粪菌干预试验4
1.2.3仔猪粪样采集4
1.2.4粪样菌群的分析4
1.2.4.1菌群DNA的提取4
1.2.4.2细菌16S rRNA基因V3-V4区序列扩增5