摘要: 血清荧光谱检测及分析方法的灵敏度很高，速度比较快，样品的制备过程也相对简便，其在肿瘤早期诊断方面的应用前景受到很大的重视，但是现有研究在诊断特异性等方面的状况尚未令人满意。本论文通过实验检测取得肝癌小鼠与健康小鼠血清的荧光光谱，研究所获取的谱线结构特征及其与癌症的相关关系。 主要研究了激发波长为280 nm 的荧光光谱数据，所研究的光谱曲线在 300 nm~400 nm 波段内具有展宽的单峰波形结构特征。22268
定性分析的结果表明，上述曲线的轮廓形态在接种了肿瘤的小鼠与健康小鼠之间有显著差异。具体表现为，肿瘤组荧光谱曲线的峰位（峰值波长）相对于健康组有显著蓝移（向短波长方向移动） ，肿瘤组光谱曲线单峰波形的上升沿比健康组更为陡峭。
对光谱曲线单峰波形的上升沿及下降沿分别作直线拟合处理，取拟合直线的斜率作为量化的指标。研究结果表明，肿瘤组光谱曲线上升沿及下降沿拟合直线的斜率绝对值显著大于健康组，拟合直线的斜率能够定量反映肿瘤组与健康组之间的差异。其数值为，肿瘤小鼠光谱曲线与健康小鼠光谱曲线上升沿拟合直线的斜率值的绝对偏差量约为3.0×105
，而两者的相对偏差量不低于50.8%。
本毕业论文的研究方法和结果，有可能为发展新的癌症早期诊断方法和技术提供有价值的参考。
关键词 血清 肿瘤 光谱 拟合 斜率
Title A study in UV-light excitation fluorescence spectra
obtained by the serum .
Abstract
Diagnosis of incipient cancer is of great significance. With high
sensitivity,high speed and relatively simple acquisition and processing
of sample, fluorescence spectrum is an effective method in the analysis
of material composition and molecular structure. The detection and
analysis methods of fluorescence spectra into the tumor detection are
of high hopes,however there are some difficulties to be solved.
This assay, the fluorescence spectra of the serum of healthy mice and liver
cancer in mice, analysis of the characteristics of its spectrum under
different excitation wavelength of peak fluorescence intensity and peak
frequency shift changes in the relationship. Excitation wavelength of 280
nm spectral data, the study of spectral curves in the 300-400 nm band
broadening unimodal structure. Experimental studies have shown that serum
discrete fluorescence intensity is relatively large . Crest curve
vaccinated group was a blue shift . Contour curve becomes steeper angle
peaks at significantly smaller trend.
This paper uses the spectral curve fitting , and healthy mice against tumors
in mice sera spectral curve analysis, and by the slope of the rising and
falling edges of its fit line to quantify the changes in the angle of the
crest . The results show that the rising and falling edge of the slope of
the curve fit line spectral intensity curve tumor group was significantly
higher than the absolute value of the healthy group . The slope of the
straight line fit to quantify the occurrence and development of tumors in
mice on a certain level .This conclusion for the use of fluorescence spectra of the possibility of
tumor diagnosis with inspiration .
Keywords serum cancer spectrum fitting slope目次
1 引言1
1.1 研究背景及意义.1
1.2 一些相关的研究及进展.2
1.2.1 国内相关的研究进展.2
1.2.2 国外对血清荧光光谱方面的一些重要的研究进展.4
1.3 本文主要的研究工作.5
2 光谱理论基础.5
2. 1 荧光原理.5
2. 1. 1 能级激发态.5
2. 1. 2 荧光的产生.6
2. 2 光影响生物组织.6
2. 3 关于荧光的一些参量.7 紫外光激发血清荧光谱分析研究:http://www.751com.cn/wuli/lunwen_14827.html