摘要：原花青素(Proanthocyanidins)，简称PC，是植物中广泛存在的一大类多酚化合物的总称，具有多种生化功效。铅是重金属中毒性较大的一种，一旦进入体内很难排除。它在体内可以诱导氧自由基的产生，而氧自由基的产生又会会诱导体内酶活力发生变化。SOD 和GPX作为源于生命体的重要活性酶类，对消除生物体内的自由基具有重要作用。胆碱酯酶(CHE)是神经传导递质代谢的一种重要的酶。本实验给小鼠饮用含醋酸铅的水，建立暴露的脑损伤模型。将小鼠分为对照组、铅暴露模型组、铅+原花青素(100 mg)组，铅+原花青素(200 mg)组、原花青素（250 mg/kg）组。实验进行3个月，结束后测定不同组脑组织SOD、GSH-PX、CHE活性。结果显示：铅抑制了脑机体SOD和GSH-PX和CHE酶的活力，这可能导致机体脑结构和功能受损。而原花青素能够抑制这些酶活力下降，呈现剂量效应。说明原花青素能够通过增加SOD和GSH-PX和CHE酶的活力，起到对铅诱导的脑损伤的保护作用。38544
毕业论文关键词：原花青素；铅；SOD；GPX；CHE；脑
Effect of Procyanidins on Activities of SOD、GPX and CHE in Mouse Liver Exposed to Lead
Abstract: Procyanidins is the generic terms of peolyphenol compound. It is distributed widely in plant and has various bio-chemical functions. Lead (Pb) is a typical heavy metal with toxicity. Once entering into human body, it will be very difficult to be get rid of. It can also induce the generation of oxygen free radicals which will further change the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and cholinesterase (CHE). SOD and GPX are of great value in eliminating the harmful metabolites. CHE is a kind of hydrolase useful in hydrolyzing acetyl choline in nerve system. In the experiment, the experimental mice are fed with lead acetate solution to establish exposed brain injury model．Mice are devided into five different groups as follows: the control group; the Pb group (250 mg/kg in dringking water); the Pb+proanthocyanidins (100 mg) group; the Pb+ proanthocyanidins (200 mg) group; the proanthocyanidins group (200mg/kg). After 3 months, the activity of SOD, GPX, CHE is measured. The results showed that Pb can inhibit the activity of SOD, GPX and CHE in brain organism and cause damage to brain’s structure and functions. However, procyanidins inhibited the change. Procyanidins can protect the brain against Pb-induced injured by increasing the activity of SOD, GPX, CHE.
Key words: Proanthocyanidins; Lead; SOD; GPX; CHE; Brain
目录
引言： 1
1.材料与方法 2
1.1 实验要用的材料 2
1.1.1实验要用的动物 2
1.1.2实验要用的试剂 2
1.1.3实验要用的设备 5
1.2 实验要做的预处理 5
1.2.1 样本要做的预处理 5
1.2.2 样本要做的采集 6
1.2.3脑组织的生化功能方面的测定 6
2.实验结果 9
2.1 SOD酶活力测定 10
2.2 GPX酶活力测定 10
2.3 CHE酶活力测定 11
2.4 结论 11
3.讨论 13
4.致谢 14
5.参考文献 15
引言
原花青素Proanthocyanidins即OPC，是一种的生物类黄酮，一般为红棕色粉末，气微、涩，溶于水和大多有机溶剂。它具有多种功效，可有效消除超氧阴离子自由基和羟基自由基，也参与磷酸、花生四烯酸的代谢和蛋白质磷酸化，保护脂质不发生过氧化损伤；是强有力的金属螯合剂，可螯合金属离子，在体内形成惰性化合物；保护和稳定文生素C，有助于文生素C的吸收和利用。原花青素是一种有着特殊分子结构的生物类黄酮的同时，更重要的意义（葡萄籽提取物）是作为一种新型高效抗氧化剂，是目前为止所发现的最强效的自由基清除剂之一[4]，具有非常强的体内活性。在抗氧化过程中，可以阻止催化氧化反应的金属离子的活性，这是它的主要生物价值所在[1]。由于OPC是水溶性，在总体吸收率上远远领先于其他物质，并且作用的范围是全身的体液。 原花青素对铅诱导脑SOD ,GPX, CHE的影响:http://www.751com.cn/yixue/lunwen_37551.html