摘要：本文以胡萝卜粉为原料，主要研究了微波处理提取、纤维素酶提取、溶剂提取这三种提取方法，通过单因素试验和正交试验，确定了这三种提取方法的最佳工艺条件，并且对这三种提取方法进行了比较。（1）用溶剂提取法提取天然胡萝卜素，在提取剂为无水乙醇，浸提温度为30℃，浸提时间为120min，浸提3次，料液比为1:20的实验条件下，天然胡萝卜素的OD值最大为0.551。（2）用微波处理提取法提取天然胡萝卜素，在提取剂为无水乙醇，辐射功率为640W，浸提时间为30min，料液比为1:10，浸提3次，天然胡萝卜素的OD值最大为0.638。（3）用纤维素酶法提取天然胡萝卜素，在提取剂为无水乙醇，酶解温度为50℃，pH值为4.0，酶用量为0.3%，酶解时间为120min，天然胡萝卜素的OD值最大为0.672。43495
毕业论文关键词： 天然胡萝卜素；提取；参数优化
Study on the extraction and stability of β carotene from carrot
Abstract： The carrot dry power as material is used in the test, mostly researched the extraction method of solvent, microwave and enzyme. The optimal parameters of these three extraction methods was identified bu single factor and orthogonal experiment, at the same time, these three methods were compared.(1) solvent extraction method was used to extract natural carotene, the extracting agent of anhydrous ethanol, leaching temperature of 30 ℃, the extraction time was 120 min, leaching 3 times, and solid-liquid ratio of 1:20 of experimental conditions, the natural carotene OD value of 0.551. (2) treated with microwave extraction to extract the natural carotene, the extracting agent of anhydrous ethanol, radiation power is 640 w, the leaching time for 30 min, and solid-liquid ratio of 1:10, leaching 3 times, natural carotene OD value of 0.638. (3) using cellulose enzymatic extraction of natural carotene, the extracting agent of anhydrous ethanol, enzyme solution temperature is 50 ℃, pH value of 4.0, enzyme dosage was 0.3%, the enzymatic hydrolysis time of 120 min, natural carotene OD value of 0.672.
Keywords: β—carotene; extraction; parameters optimization
目录
1 绪论 1
1.1 天然胡萝卜素的功能 1
1.1.1 天然胡萝卜素是维生素A的重要来源 1
1.1.2 天然胡萝卜素具有抗氧化作用 2
1.1.3 天然胡萝卜素可以促进细胞缝隙间的连接交流 2
1.1.4 天然胡萝卜素可以增强人体免疫力 3
1.1.5 天然胡萝卜素的其他功能 4
1.2 天然胡萝卜素的市场概况 4
1.3 天然胡萝卜素的生产方法 5
1.3.1 化学合成法 5
1.3.2 从天然植物中提取 5
1.3.3 由藻盐培养 5
1.3.4 微生物发酵法 5
1.4 本课题的目的及研究意义 6
2 实验材料与方法 7
2.1 实验材料 7
2.2 实验试剂 7
2.3 仪器与设备 7
2.4 工艺流程 7
2.5 溶剂提取试验 8
2.5.1 溶剂提取单因素试验 8
2.5.2 溶剂提取正交试验 胡萝卜色素提取工艺条件研究:http://www.751com.cn/shiping/lunwen_44362.html