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摘要：弗吉尼亚栎（Quercus virginiana)别名强生栎，为壳斗科栎属常绿乔木，原产美国。耐盐碱，抗风力强，是沿海防护林和城市园林优良树种。为给弗吉尼亚栎的引种提供理论依据，以弗吉尼亚栎成熟叶片和茎为材料，采用电导率法对，结合Logistic方程拟合其细胞伤害率的曲线，以确定弗吉尼亚栎的低温、高温半致死温度。结果表明，随着温度的降低或升高，弗吉尼亚栎叶片和茎细胞的伤害率均呈现“S”型变化，通过显著性验证，符合Logistic方程。通过方程拐点确定弗吉尼亚栎的叶片和茎的低温半致死温度分别为-9.4℃和-49.4℃，叶片和茎的高温半致死温度分别为41.8℃和47.2℃。表明弗吉尼亚栎有较强的低温、高温耐受性。20832
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关键词：弗吉尼亚栎；半致死温度；Logistic方程；相对电导率
Semi-lethal temperature of Quercus virginiana
Abstract: Live oak (Quercus virginiana Mill.) is a large-statured, broad leaf, evergreen hardwood species native to the southeastern United States. It's an important greening tree in urban area and coast protection forest because of its resistance to salt-alkaline and strong wind. In order to offer a theoretical basis for introduction of Quercus virginiana, taking mature leaves and stems in Quercus virginiana as materials, cold tolerance and heat tolerance were researched by electrical conductivity method, and semi-lethal high and low temperatures were calculated by fitting the cell injury rate curve combined with Logistic equation. The results indicate that the curve of cell injury rate in Quercus virginiana showed a typical“S”shape with temperature decreasing or increasing, and fitted Logistic model significantly. The semi-lethal low and high temperatures of the leaves of Quercus virginiana, determined by the inflection point of curve, are -9.4℃, 41.8℃, while the semi-lethal low and high temperatures of the stems of Quercus virginiana are -49.4℃, 47.2℃. It indicated that Quercus virginiana is tolerant to low or high temperature stress.
Key words: Quercus virginiana; Logistic equation; semi-lethal temperature; relative electrical conductivity
目 录
1 绪论 1
1.1 弗吉尼亚栎 1
1.1.1 栎属综述 1
1.1.2 弗吉尼亚栎生物学特性 1
1.1.3 弗栎栽培繁殖研究 2
1.1.4 弗栎在美国原生地城市绿化中的运用 2
1.1.5 在我国的发展前景: 4
1.2 半致死温度研究意义 5
1.3 应用logistic方程测定植物半致死温度 5
1.3.1 应用logistic方程测定植物半致死温度的原理 5
1.3.2 应用logistic方程测定植物半致死温度的拟合方法 6
1.3.3 应用logistic方程测定植物半致死温度研究在国内外的进展 6
1.4 测定弗吉尼亚栎高温、低温半致死温度的意义 7
2 材料与方法 7
2.1 试验材料 7
2.1.1 试验材料与处理 7
2.1.2 试验时间及地点 8
2.1.3 试验主要应用仪器 8
2.2 试验方法 8
2.2.1 低温胁迫下弗吉尼亚栎半致死温度测定 8
2.2.2 高温胁迫下弗吉尼亚栎半致死温度测定 9
2.2.3 Logistic方程拟合和半致死温度计算 9
2.2.4 半致死温度下叶绿素荧光参数变化 9
3 结果与分析 9
3.1不同低温胁迫下弗吉尼亚栎电解质外渗率的动态变化 9
3.1.1 不同低温胁迫对弗吉尼亚栎叶片电解质外渗率的动态变化 9