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摘要：棉花黄萎病现已成为限制棉花生产的重要病害之一，严重影响棉花的产量和品质，目前还没有有效的防治方法。该病害为土传真菌性文管束病害，其致病病原菌为黑白轮枝菌（Verticillium albo-atrum）和大丽轮枝菌（Verticillium dahlia Kleb）两种，而我国棉花产区的病原菌多属大丽轮枝菌（V. dahliae）。棉株受到大丽轮枝菌侵染后，其自身的组织结构会发生一系列变化来抵抗病原菌的侵入，在与病原菌中互作过程中会发生一系列防御反应抵挡病原菌的进一步入侵。本研究使用具有绿色荧光标记的大丽轮枝菌中等致病力菌系来侵染6种不同抗性的棉花品种，研究大丽轮枝菌侵染不同抗性棉花品种过程中产生的防御反应的差异。研究发现，棉花的黄萎病抗性越强，其防御反应程度也越强，表现在接种大丽轮枝菌后，棉花各个部位的大丽轮枝菌的生物量随品种抗病性的增强而减少，根部的木质素含量、叶片胼胝质含量和过氧化氢的积累量都随抗病性的增强而增多，防御酶如多酚氧化酶（PPO）、过氧化物酶（POD）和过氧化氢酶（CAT）活性的升高幅度随抗病性的增强而增大。28124
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关键词：棉花；黄萎病；大丽轮枝菌；防御反应
Research Advances in the Interaction Mechanism Between Cotton and Verticillium dahliae
Abstract： Cotton Verticillium has become one of the critical diseases to restrict the cotton production, which lead to serious loss in yield and fiber quality. Moreover, there isn’t an efficient way to control the disease. This disease is a soil-borne and seed-borne vascular disease, the main causative pathogen is Verticillium albo-atrum and Verticillium dahlia. While the majority pathogenic bacteria is Verticillium dahliae in Chinese cotton-producing areas. After the infection by Verticillium dahlia, the tissue structure of cotton will response with a series of changes to resist the invasion of pathogens, and in process of the interection with pathogens the cotton will produce a series of defense reactions to resist further invasion of pathogens. This study adopted the Verticillium dahliae which marked by green fluorescent protein (GFP) to effect six different varieties of cotton, study on the responses when sdifferent resistance cotton varieties was infected by Verticillium dahlia. Research showed that the immune response was related to the resistance enhancement after Verticillium dahliae infection, different varieties of cotton were inoculated with Verticillium dahliae spore suspension, the resistances of cultivars were evaluated. The Verticillium dahliae biomass indifferent parts of cotton were negatively related with resistance level, and lignin content of hypocotyl, leaves callose deposition and hydrogen peroxide accumulation increased with the enhancement of disease resistance, the defensive enzymes activity like polyphenol oxidase (PPO), peroxidase (POD) and catalase (CAT) were positively related with disease resistance.
Key words: Cotton; Verticillum wilt; Verticillium dahlia; Defense response
摘要1
关键词1
Abstract1
Key words1
引言（或绪论）2
1 材料与方法2
1.1 实验材料 2
1.1.1供试材料与菌株2
1.1.2实验仪器2
1.1.3实验试剂2
1.2 实验方法 2
1.2.1培养棉苗2
1.2.2大丽轮枝菌孢子悬浮液制备2
1.2.3不同棉花品种的黄萎病抗性鉴定3
1.2.4大丽轮枝菌侵染的显微观察以及生物量检测3
1.2.5木质素测定3
1.2.6胼胝质检测4
1.2.7过氧化氢（H2O2）测定4
1.2.8酶活性测定4
1.2.9数据分析4
2 结果与分析5
2.1不同棉花品种的黄萎病的抗性5
2.2 植株中大丽轮枝菌的生物量5
2.3大丽轮枝菌对棉花根部木质素含量的影响6
2.4大丽轮枝菌处理后棉株叶片的胼胝质沉积6
2.5叶片过氧化氢积累7