-
两种典型环境污染物的水生植物毒性研究摘要：强力霉素和草甘膦在全世界范围内用量广泛，它们的残留物最终都会通过降雨，地表径流和渗透等不同途径进入水体中，是两种环境中检出率较高的污染物。近年来，由于水体中爆发的蓝藻水华成为了世界性的环境问题。然而，这两种典型环境污染物对蓝藻的生理效应研究还不足。本研究以铜绿微囊藻和绿色微囊藻作为非靶标生物，以此来探究强力霉素和草甘膦对水生植物的生态毒性。结果表明，在实验条件下，强力霉素和草甘膦对两种微囊藻的生长产生抑制作用，也诱发了不同程度的氧化损伤。草甘膦对两种微囊藻叶绿素a和蛋白含量的合成也有一定的刺激作用。与此同时，草甘膦还致使藻细胞发生早期凋亡，藻细胞的形态结构发生变化。本文的研究结果对两种典型环境污染物的环境评估有一定的参考意义。此外，对强力霉素和草甘膦在农业方面的合理使用也起到一定的引导作用。29327
- 上一篇：一氧化氮对拟南芥愈伤组织耐热性的影响
- 下一篇：油菜素内酯对大豆与疫霉菌互作中活性氧清除能力的研究
毕业论文关键词：强力霉素；草甘膦；铜绿微囊藻；绿色微囊藻；氧化损伤
Aquatic Toxicity of Two Typical Environmental Pollutants
Abstract: Doxycycline hydrochloride and glyphosate have been used extensively worldwide. The residues of them can be discharged into water system through different exposure routes such as raining, surface runoff and infiltration, these two pollutants have high detection rate at environmentally relevant concentrations. Water blooms caused by cyanobacteria in aquatic system are major global environmental issues in recent years. However, the physiological effects on cyanobacteria caused by these two typical environmental pollutants are insufficient. In the present study, Microcystis aeruginos and Microcystis viridis were selected as non-target organisms to explore the ecotoxicity of doxycycline hydrochloride and glyphosate. The results showed that these two typical environmental pollutants could inhibit the growth of Microcystis aeruginos and Microcystis viridis under laboratory conditions and they could also induce oxidative damage. Glyphosate could stimulate the protein and chlorophyll a content of Microcystis aeruginos and Microcystis viridis. At the same time, glyphosate could result in early apoptosis of cells and the cellular structure has been changed. The results are useful for environmental assessment of these two typical environmental pollutants. Besides, it is also helpful for guiding the application of doxycycline hydrochloride and glyphosate in agricultural settings.
Key Words: doxycycline hydrochloride；glyphosate；Microcystis aeruginos；Microcystis viridis；oxidative damage
目录
1引言 1
1.1两种典型污染物的污染现状 1
1.1.1抗生素 1
1.1.2除草剂 1
1.2两种典型污染物的生态毒性 2
1.2.1强力霉素 2
1.2.2 草甘膦 3
1.3两种微囊藻概述 4
1.4 课题研究的意义与内容 5
2实验条件及依据 7
2.1实验用品 7
2.1.1实验仪器 7
2.1.2实验材料 7
2.1.3实验药品 8
2.2实验前期准备 9
2.2.1培养液的配置 9
2.2.2高压蒸汽灭菌 9
2.2.3微囊藻的接种 10
2.2.4微囊藻的培养 10
2.2.5强力霉素储备液的配制 10
2.2.6草甘膦储备液的配制 10
2.3实验方案论证 10
3两种污染物对两种藻的生长效应研究 12
3.1藻细胞数与吸光度的关系 12
-
常温土壤环境中嗜热香草...
-
高中生环境意识培养现状及策略研究
-
低pH环境下小麦生长发育的蛋白表达变化研究
-
洪泽湖地区典型地物NDVI变化的时序分析
-
水稻和拟南芥BRD结构域非...
-
玉米BRD结构域非典型激酶...
-
环境和生物样品中3H样品的制备与处理
杂拟谷盗体内共生菌沃尔...
中考体育项目与体育教学合理结合的研究
java+mysql车辆管理系统的设计+源代码
当代大学生慈善意识研究+文献综述
电站锅炉暖风器设计任务书
乳业同业并购式全产业链...
大众媒体对公共政策制定的影响
十二层带中心支撑钢结构...
酸性水汽提装置总汽提塔设计+CAD图纸
河岸冲刷和泥沙淤积的监测国内外研究现状