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摘要：本文采用FLS900型稳态和瞬态荧光光谱仪研究了银纳米颗粒对全血溶液的荧光增强效应。首先，研究了不同浓度全血溶液的荧光光谱，发现全血溶液在512nm和560nm处有荧光峰。其次，在不同浓度的血液中加入相同量的银纳米颗粒进行荧光增强研究，发现血液的荧光峰强度得到明显增强，其中512nm 处的荧光强度的增强倍率为7.3，560nm 的荧光强度的增强倍率为11.9。实验结果表明银纳米颗粒能提高荧光的发射效率，显著增强血液荧光。最后，将不同浓度的荧光发射峰的荧光增强倍数进行了对比，发现当血液浓度为0.2%时，荧光增强效果最强。分析认为，银纳米粒子和血液中的荧光生色团互相作用产生了荧光增强效应，而增强效果和银纳米粒子与荧光物质之间的距离密切相关。34363
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本文的研究结果为银纳米颗粒对全血溶液的荧光增强作用提供了实验依据，对后续的研究具有一定的参考价值。
毕业论文关键词：荧光光谱；荧光增强；银纳米颗粒；血液；最佳浓度
Study on fluorescent enhancement effect of silver nano particles on whole blood solution at different concentrations
Abstract: The FLS900 steady-state fluorescence spectrometer was used to study fluorescence enhancement effect of silver nano particles on whole blood solution at different concentrations. Fluorescence spectra of whole blood solution at different concentrations was investigated firstly, and fluorescence peak is 512nm and 560nm. The fluorescence intensity increased obviously when a certain amount of silver nano particles were added in the blood. The multiplying power of fluorescence intensity at wavelength of 513nm and 560nm is 7.3 and 11.9, respectively. The result showed that silver nano particle can enhance fluorescence intensity and fluorescence emission productiveness obviously. The multiplying power of fluorescent emission peak at different concentrations was compared and the best fluorescence enhancement effect was observed at the concentration of 0.2%. It was considered that the alternative effect of silver nano particle and fluorescent chromophore led to fluorescence enhancement effect, which is closely related to the distance between silver nano particles and fluorescent chromophore.
The conclusion of this paper provided practical basis of fluorescent enhancement effect of silver nano particles on whole blood solution at different concentrations, it can offer reference to related research in the future.
Keywords: Fluorescent spectra, Fluorescence enhancement effect, Silver nano particle, Blood, Best concentration,
目录
摘要 i
Abstract ii
1 绪论 1
2 荧光光谱理论 2
2.1 荧光光谱的产生 2
2.2 激发光谱和发射光谱 3
2.3荧光光谱的主要参量 4
2.4荧光分析法的优点 4
2.5血液的荧光光谱 5
3 表面增强荧光效应 9
3.1 表面增强荧光现象 9
3.2 金属纳米颗粒增强荧光效应 9
3.3 银纳米颗粒的荧光增强效应 10
4 实验仪器和样品 11
4.1 实验装置和仪器 11
4.2 实验样品的制备 12
5 实验方法与结果 13
5.1 全血溶液的荧光光谱 13
5.2 加入银胶的全血溶液荧光光谱 15
6 实验分析与讨论 17