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摘 要: 我国银杏叶资源十分丰富，银杏叶中含有重要的中药成分银杏黄酮，但长期以来，银杏叶都被人们忽视，造成一定的资源浪费。为了能从银杏叶中提取出更多的银杏黄酮，本论文就银杏黄酮提取条件进行优化。针对银杏黄酮，采用微波助提的方式，通过研究固液比、乙醇浓度、提取时间、提取温度四个方面的研究，获得银杏黄酮提取的最优条件。其中，银杏叶片总黄酮提取的最优条件为：70%乙醇、1：30的料液比、提取温度37℃、提取时间30min，再经150W微波辅助提取4min，最终银杏叶片总黄酮提取浓度为0.2552mg/ml。银杏叶梗总黄酮提取的最优条件为：60%乙醇、1：30的料液比、提取温度45℃、提取时间15min，再经200W微波辅助提取2min，最终银杏叶梗总黄酮提取浓度为0.1346mg/ml。通过上述研究，能够为银杏叶总黄酮的工业化提取提供工艺技术参考。57999
毕业论文关键词：银杏叶；银杏梗；黄酮；提取；微波
Abstract：There are abundant resources of Gingko leaves in our country, and which contains Ginkgetin, a kind of significant Chinese herbaled medicinal ingredient. However, people had neglected them which caused a waste of resources for a long time. In order to extract more ginkgetin from Gingko leaves,so I wanted to do the subject as the optimization of Gingko Leaves Extraction Conditions. According to Ginkgetin, I took the means of microwave-assisted extraction by studying the following four aspects, solid-to-liquid ratio, ethanol concentration and the time and temperature of extraction.In the end, I obtained the optimization conditions of gingko leaves extraction. The optimization condition of gingko leaves extraction was: 70% ethanol, the ratio of solid material to liquid 1:30, 30 min extraction at 37℃, 150 W microwave extraction 4 min, The concentration of Flavone were 0.2552mg/ml; The optimization condition of Gingko biloba leaf stalks extraction was: 60% ethanol, the ratio of solid material to liquid 1:30, 15 min extraction at 45℃, 200 w microwave extraction 2 min, The concentration of Flavone were 0.1346mg/ml. Through the above research, we can provide industrialized utilization of extraction of flavonoids from ginkgo biloba leaves technology reference.
Keywords: Gingko leaves; Ginkgetin; Flavone; extract; microwave.
目 录
1. 前言 5
1.1 选题背景 5
1.2 本课题的研究意义 5
1.3 银杏简介 5
1.4 银杏黄酮的作用 5
1.5 应用现状及发展前景 6
1.6 银杏黄酮开发利用的问题与对策 6
1.7 论文设计思路 6
2. 材料与方法 7
2.1 实验材料 7
2.1.1 原料 7
2.1.2 试剂 7
2.1.3 仪器 7
2.2 实验方法 7
2.2.1 主要试剂配置 7
2.2.2 原料的处理 8
2.2.3 实验原理 8
2.2.4 标准品的选择及最大吸收峰的确定 8
2.2.5 制标准曲线 8
2.2.6 计算方式