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摘 要:非生物胁迫如寒害、旱害、盐害、病虫害等对植物的生长发育具有较大程度的危害，而严重的非生物胁迫对油菜等作物会造成严重的经济损失,从而影响作物的生产。植物在非生物胁迫下，会诱导产生多种蛋白以适应胁迫压力，其中 SAP（stress-associated protein） 蛋白在胁迫应答和胁迫调控中具有重要作用而被广泛关注。因此不断挖掘与鉴定新的胁迫相关蛋白是植物学研究的热点之一。为了获取油菜中胁迫相关的蛋白信息，本研究对 NCBI蛋白质序列数据库进行检索获得27条白菜型油菜胁迫相关蛋白序列，并运用生物信息学技术及方法对该家族蛋白序列的基本信息、结构、定位、蛋白序列保守域和进化信息等进行了分析。结果表明，油菜SAP蛋白家族共27条序列（除去一致性序列），分布在第Ⅰ至Ⅸ条染色体上，其中第9号染色体上SAP基因位点最多，为7个基因位点，基因位点最少的染色体是1号和4号染色体；所有蛋白序列成员中19个定位于细胞核中，有3个成员定位于细胞外，5条同时定位于细胞核和线粒体中；该家族成员对应基因具有1-4个外显子；按保守结构域不同将其分为三类，其中第一类4个成员，具有RAMP4结构域特征，第二类1个成员，具有zfA20结构域系列特征，第三类分别具有22个成员，同时具有ZnF-AN1和zfA20结构域序列特征。进化分析表明，SAP蛋白具有较多异变位点，多序列比对的差异性大，在进化过程中易发生基因突变来适应胁迫环境。本研究为利用相关数据开发油菜的相关抗逆新品种奠定理论基础。34529
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毕业论文关键词：油菜；SAP蛋白；基因结构；进化分析
Discovery and Identification of Stress-associated Protein of SPA Family Members in Brassica Rape L
Abstract: Abiotic stresses such as chilling injury, damage of drought, salinity and plant diseases and insect pests, is harmful to plant growth and development. Greater degree of harm and serious abiotic stress will be caused serious economic losses on rape and other crops, which affecting crop production. With abiotic stresse, crops would be induced to produce varieties of protein to adapt to the stress pressure, the SAP (stress-associated protein) proteins induced by abiotc stress have important roles on responses of stress and the regulation of stress. Therefore, it is one of the hot topics in botanical research to tap and identify new stress-related proteins. In order to obtain the information of stress-associated protein of rape. This study were retrieved 27 different stress-associated protein sequences of cabbage from NCBI protein sequence database, then by biological information technology and the methods, we got basic physical and chemical information, structure information, location, protein sequences conserved domains and evolutionary information. The results showed that, the SAP protein family in rape has 27 diferent members (excluding consistent sequences), distributed in from I to IX chromosomes, the number of SAP gene sites on ninth chromosome has 7 genetic locus, the genetic locus in the 1th and 4th chromosome was the fewest. In all SAP stress-association protein members, 19 members located in the nucleus, 3 members located in extracellular, 5 members located in both nucleus and mitochondria; the family members have 1-4 exons; according to the different conserved domains, it will be pided into three categories, the first category has 4 members, which have RAMP4 domain, the second only has 1 member, with the zfA20 domain, the third type have 22 members, with both ZnF-AN1 domain and zfA20 domain. Evolutionary analysis showed that the SAP protein had many mutation sites, and the difference of multiple sequence alignment was larger, and the gene mutation was prone to adapt to the stress environment during the evolution. This research is to lay the theoretical foundation for the development of rape new varieties– resisitance to stress.