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摘要：TIFY转录因子超家族是在植物中特有的基因家族，在植物的生长与发育过程中起重要作用。目前，在模式植物拟南芥中对TIFY家族基因的研究比较多，但在茄科植物中有关TIFY家族基因的研究却很少。本文以番茄与土豆作为茄科植物代表，基于计算生物学方法对这2个物种的TIFY基因进行系统性剖析。系统发生与序列分析表明，21个番茄和21个土豆TIFY基因被分为4个类群，每个类群内具有相似的基因结构与保守基序组成，但不同类群之间的基因结构与蛋白序列特征有明显分化。对番茄与土豆的2对旁系同源基因的选择压力和表达谱分析显示：2对旁系同源基因均受控于负选择，具有较高保守性，未发生适应性进化；但2对旁系同源基因的表达谱发生了明显分化，说明这2对同源基因的分化主要体现在基因表达谱上。这些研究结果将有助于我们进一步深入研究茄科植物TIFY基因的生物学功能，也为深刻认识TIFY蛋白在茉莉酸信号转导途径中的作用奠定理论基础。 36160
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毕业论文关键词：茄科植物；TIFY家族；基因；转录因子；进化
Molecular Identification and Comparative Analysis of the TIFY Family Genes in Solanaceae Species
Abstract: TIFY transcription factor superfamily in plants specific gene family plays an important role in the growth and development of plants in the process. At present, the model plant Arabidopsis to study TIFY family genes are more, but in the Solanaceae plant family genes related TIFY research are few. In this paper, tomato and potato as a representative of the Solanaceae, based on computational biology methods of these two species TIFY systematically analyze gene. Phylogenetic and sequence analysis revealed that 21 tomato and 21 potato TIFY genes were pided into four groups, each groups have similar gene structure and conserved motifs, but the structure and characteristics of the protein sequence of the gene between different groups There are obvious differentiation. For two pairs of paralogs tomato and potato selection pressure and expression profiling analysis showed: two pairs of paralogs are controlled by negative selection, with high conservation of adaptive evolution did not occur, but two pairs paralogs gene expression profile distinct differentiation occurs, indicating that the two pairs of homologous genes differentiation is mainly reflected in the gene expression profiles. These findings will help us to further study the biological function of the Solanaceae TIFY genes, but also for a deep understanding of the role TIFY protein in jasmonate signal transduction pathway theoretical basis.
Key words: Solanaceae, TIFY family, Gene, Transcription factor, Evolution
目 录
摘要 1
引言 2
1 数据与方法 3
1.1 基因检索与数据下载 3
1.2 基因结构分析 3
1.3 蛋白保守基序与结构域分析 3
1.4 序列联配与系统进化树构建 4
1.5 同源基因对的选择压力检测 4
1.6 同源基因对的表达分歧度分析 4
2 结果与分析 4
2.1 茄科植物TIFY家族基因的鉴定 4
2.2 茄科植物TIFY家族基因的结构特征 5
2.3 茄科植物TIFY蛋白的保守基序组成模式 7
2.4 茄科植物TIFY基因的系统进化关系 8
2.5 茄科植物TIFY同源基因的选择压力 10
2.6 茄科代表物种特异扩增同源基因对的表达情况 10
3 讨论与结论 11
参考文献 13
附录 14
致谢 15
茄科植物TIFY家族基因的鉴定与比较分析引言