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摘要： 姜黄素具有较多药理活性，包括抗氧化、抗凝血、抗炎、降血脂、延缓衰老及抗癌等。有关于姜黄素的药物制剂研究越来越广泛，但由于其在体内的生物利用率不高，因此本文选取V-型直链淀粉作为壁材来包埋姜黄素，并对其包埋率，溶解度，稳定性进行研究。结果表明：使用制备的V-型直连淀粉，能够将姜黄素均匀包埋，得到良好的V-型直链淀粉包合物，制备的V-型直链淀粉包合物进行XRD,DSC,电镜扫描及TGA的表征，XRD表征结果表明，用V-型直链淀粉分子将姜黄素包合，形成新的物相。DSC表征结果可以得知，V-型直链淀粉与姜黄素之间所形成的分子包合物共吸热焓最大，预示着V-型直链淀粉姜黄素包合物具有较优的包合特性。电镜扫描结果无法直接显示出包合物与其它物象之间存在的区别，TGA结果可以说明姜黄素被包埋之后提高了姜黄素的热稳定性。制备过程在避光状态下进行，其中V-型直链淀粉与姜黄素的质量比为2:1，包埋时间为1个小时，温度为70℃，油浴锅中进行。此外，利用紫外分光光度法测定的包合物的包埋率达40%，经过热稳定性及光稳定性实验证明将姜黄素包埋于V-型直链淀粉中有助于提高姜黄素稳定性。43515
毕业论文关键词： V-型直链淀粉；姜黄素；包合物；包埋率；稳定性
Preparation of curcumin-V- type amylose inclusion complex and the research of Stability
Abstract：Studies had showed that curcumin has efficacious pharmacological activities, such as antioxidant, anticoagulant, anti-inflammatory, lowering blood pressure, anti-aging and anti-cancer. The research of the pharmaceutical has been more and more widely. But in many ways, the bioavailability in the body is not so well, so this paper selected V- type amylose as wall material to encapsulate the curcumin, to improve its performance. At the same time, explored the determination of embedding rate, solubility and stability. The results showed that: in the preparation of the V-type amylose, the curcumin solution dropped in it directly and carry on complex reaction. This method can make curcumin entrapped more uniform, and got a good V – type amylose straight chain starch package compound. The inclusion complexes were confirmed by X-ray diffraction (XRD). The rmogravimetric analysis (TGA) and differential scanning calorimetry (DSC) showed that the inclusion reaction of amylose with curcumin yielded the most stable complex. The preparation process is carried out in a dark, core wall ratio is 2:1, the embedding time for 1 hour, the temperature is70℃.And the embedding rate measured by UV spectrophotometry was 40.00%, it was helpful to improve the stability of curcumin according to the thermal stability and light stability test.
Key words: V-type amylose, curcumin, inclusion complexes, complexation efficiency, stability
目录
1 绪论 1
1.1 姜黄素简介及其功能 1
1.1.1 抗氧化作用 1
1.1.2 促进细胞凋亡 1
1.1.3 抑制肿瘤细胞生长和转移 1
1.1.4 阻断肿瘤细胞的信号传导通路 1
1.1.5 调节钙离子 2
1.1.6 抑制肿瘤血管生成 2
1.1.7 抑制炎症的作用 2
1.1.8 抗病毒作用 2
1.2 V-型直链淀粉概述 2
1.3 姜黄素制剂国内外研究进展 3
1.4 以V-型直链淀粉为壁材的包合物的研究进展