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摘要：表面增强拉曼（SERS）光谱技术广泛应用于多个领域，如生命科学、材料科学、催化化学与环境化学等研究领域。而利用基于纳米粒子的SERS光谱技术用于研究生物活性分子在纳米材料界面的作用方式则结合了SERS光谱技术和纳米粒子的诸多优点，大大推进了SERS光谱技术在涉及生物分子研究领域应用的广度与深度。因此，本论文制备了稳定性好、灵敏度高的SERS基底材料，用于探测多巴胺及类似物在不同纳米材料表面的SERS活性。该论文以多巴胺、L-去甲肾上腺素和3，4-二羟基苯乙酸为目标分子，通过电化学方法及SERS光谱技术研究了不同pH值对多巴胺及类似物在纳米银基底表面结合方式的影响。该论文为进一步研究与纳米材料作用的生物分子的结构与性质及分子与纳米材料表面之间的结合状态与作用机理奠定了基础，且对于深入理解生物分子结构与其功能间的关系具有十分重要的意义。关键词: 表面增强拉曼光谱（SERS）；pH值；银纳米颗粒；多巴胺及类似物8814
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The influence of the solution’s acidity and alkalinity on bioactive molecule in nano-material interface configuration
Abstract: The Surface Enhanced Raman Scattering (SERS) technique has been widely used in many research fields, such as life science, material science, catalysis, and environment chemistry. Utilization of the SERS technique based on nano-particles for studying the biological molecules can incorporate very effectively both the advantage of SERS spectroscopy and those of nano-particles. It can greatly broaden and go deep into the applications of SERS technique in research field concerning biological molecules. For this purpose, our research work prepares the SERS substrate materials having good stability, for detecting the SERS activity of the dopamine in different nano-material surface. Dopamine, L- nor epinepHrine and 3, 4-dihydroxypHenylacetic acid were selected as the representative molecules for our research. Through the electrochemical method, the ultraviolet spectrum and SERS spectra technology study the effect of the pH values on dopamine in different metal nano-particles. Lay the foundation for the mechanism of combining state and interactions between biological molecules to further research and nanometer material effect of structure and properties and molecular and metal surface. It has very important significance for understanding the relationship between molecular structure and its function.
Keywords：Surface Enhanced Raman Scattering；pH value；metal nanoparicles；Dopamine
目录
1 前言 1
1.1 表面增强拉曼散射（SERS）的介绍 1
1.1.1 拉曼散射的基本原理及特点 1
1.1.2 SERS现象及原理 2
1.2 金属纳米材料及其在SERS光谱中的研究 3
1.2.1 纳米材料简介 3
1.2.2 纳米材料的特性 4
1.2.3 纳米材料的制备方法 5
1.2.4 SERS基底的制备及应用现状 5
1.2.5 SERS光谱的应用研究 6
1.3 电化学研究 7
1.4 多巴胺及类似物简介 8
1.5 课题的目的和意义 9
2 实验部分 9
2.1 试剂和仪器 9
2.1.1 试剂 9
2.1.2 仪器 10
2.2 实验步骤 10
2.2.1试剂的制备 10
2.2.2不同pH值下PBS缓冲溶液的配制 10
2.2.3 纳米银基底的制备 11
2.2.4银纳米探针的制备 11
2.2.5电化学测试 11
2.2.6表面增强拉曼光谱测试 11
3 实验结果和讨论 11