桦褐孔菌伴生菌对几种食药用菌生长的影响

菜单

摘要：桦褐孔菌具有重要的药理作用，应用范围广泛，其人工栽培生长周期长，活性物质含量少。为提高其生长速度和产量，我们从桦褐孔菌菌核中分离得到了8株伴生菌，研究这8种伴生菌对桦褐孔菌以及灵芝、杏鲍菇和侧耳等食药用菌种生长情况的影响。通过探究结果表明：桦褐孔菌伴生菌Z对于这四种食药用菌种的生长都具有一定的促进作用，对于桦褐孔菌、灵芝、杏鲍菇与侧耳的生长分别提高12.2%、2.2%、14.8%和2.1%。桦褐孔菌伴生菌Q、伴生菌J、伴生菌M、伴生菌O、伴生菌C、伴生菌F对于四种食药用菌的生长具有不同的促进和抑制作用。最后，通过对伴生菌Z促生物质进行分离鉴定，获得具有促生化合物的物质，通过核磁和质谱分析，鉴定为半乳糖醇。69045

毕业论文关键字：桦褐孔菌，桦褐孔菌伴生菌，灵芝，杏鲍菇，侧耳，生长影响

Effects of Inonotus obliquus companion fungus on some edible and medicinal fungis growth situation

Abstract: Inonotus obliquus has important pharmacological effects and a wide range of applications. The artificial cultivation has long growth cycle and low content of the bioactive substances. In order to improve the growth rate and yield,we got eight companion fungus from Inonotus obliquus sclerotium.We studied the effects of 8 fungus on the growth of edible and medicinal fungi,such as Ganoderma lucidum ，Pleurotus eryngii and Pleurotus ostreatus. The research results show that: strain Z for the growth of the four kinds of edible and medicinal fungi have a certain role in promoting, for the growth of Inonotus obliquus, Ganoderma lucidumPleurotus eryngii and Pleurotus ostreatus were increased by 12.2%, 2.2%, 14.8% and 2.1%. Strain Q and strain J, strain M, strain O, strain C and strain F for the growth of the four kinds of edible and medicinal fungi have different promotion and inhibition. Finally, Associated fungus Z were galactitol. The compounds were identified by NMR and mass spectrometry.

Keywords: Inonotus obliquus(Fr.) ，Inonotus obliquus companion fungi，Ganoderma lucidum ，Pleurotus eryngii ，Pleurotus ostreatus，Growth effect.

绪论 1

1. 材料与方法 2

1.1：材料 2

1.1.1四种食用药用菌类 2

1.1.2桦褐孔菌伴生菌类 3

1.2：实验仪器（主要） 3

1.3：培养基 3

1.4 实验方法 5

1.4.1总体流程 5

1.4.2具体步骤 6

2. 结果与分析 7

2.1桦褐孔菌组实验结果与分析 7

2.2灵芝组实验结果与分析 9

2.3杏鲍菇组实验结果与分析 10

2.4侧耳组实验结果与分析 11

2.5伴生菌z促生化合物的分离和鉴定 12

3.讨论 15

3.1.实验结果讨论： 15

3.2.问题与解决方案 15

3.3.关于实验环境 15

4. 致谢 16

5.参考文献 16

绪论

桦褐孔菌[Inonotus obliquus(Fr.)] 是十分珍稀而又名贵的食药用真菌。主要分布在北半球北纬40°～50°的地区，如北美、芬兰、波兰、俄罗斯、中国、日本等部分地区。在我国，桦褐孔菌资源主要分布在吉林长白山区、黑龙江大兴安岭、内蒙古等地[1]。其药用部位为桦褐孔菌的菌核，菌核中主要含有羊毛甾醇型三萜类、桦褐孔菌醇、桦褐孔菌素、黑色素类、叶酸衍生物以及芳香物质、木质素、栓菌酸、单宁等化学成分,其中起主要药理作用的为羊毛甾醇型三萜类、桦褐孔菌醇、桦褐孔菌素[2] 。具有抵抗肝癌、胃癌、肺癌、宫颈癌、乳腺癌、皮肤癌、直肠癌、淋巴等多种肿瘤细胞,以及抗艾滋病病毒、抗感染、治疗糖尿病、降血压调节血脂、抗机体衰老、增强免疫力等药理作用。目前，桦褐孔菌已经能够人工栽培，但人工栽培产量低，活性物质含量少，抗肿瘤抗氧化活性明显低于野生菌核[3][4]。论文网