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摘要：实验用FLS900光谱仪测定出半边莲和半枝莲的激发光谱以及不同浓度下二者的发射光谱和荧光偏振光谱，得到二者的光谱曲线变化规律，研究对比半边莲与半枝莲的荧光光谱，结果显示，半边莲对450nm的光较敏感，半枝莲对460nm的光较敏感；相同浓度时，两种中药荧光特性差异较大；不同浓度时，半边莲的发射峰位于520nm-525nm之间，半枝莲的发射峰位于532-542nm之间，两种中药的荧光偏振光谱差别较大，半边莲和半枝莲可以用荧光光谱法区分。38360
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毕业论文关键字： 荧光光谱 偏振 中药鉴别
Comparative study on spectral characteristics of polarization of lobelia and barbed skullcap herb
Abstract: Experimental determination of lobelia and barbed skullcap herb in different concentrations get the excitation spectrum , emission spectra and fluorescence polarization spectrum ,in which shows that the change rule of spectral curve .FLS900 fluorescence spectrometer was used to contrast the study of lobelia and fluorescence spectrum in this project. Results show that the lobelia is sensitive to the light of 450nm, barbed skullcap herb is sensitive to the light of 460nm;The two kinds of traditional Chinese medicine (TCM) fluorescence features are different in the same concentration;The emission peak of lobelia is between 520nm and 525nm the emission peak of barbed skullcap herb is between 520nm and 525nm,the change rule shows a greater difference between two polarization spectrum of traditional Chinese medicine (TCM). lobelia and barbed skullcap herb can be pided efficiently using the method of fluorescence analysis .
Key words: Fluorescence spectrum fluorescence polarization identification of traditional Chinese medicine ;
目录
摘要 I
Abstract II
1绪论 1
1.1中药常用鉴别方法 1
1.2材料样品 2
1.2.1半边莲 2
1.2.2半枝莲 3
1.3光谱分析技术 3
1.4荧光激发光谱和发射光谱 4
1.4.1荧光激发光谱 4
1.4.2荧光发射光谱 4
1.5荧光的偏振度 5
2半边莲和半枝莲荧光光谱测量 5
2.1测量仪器及测量技术 5
2.1.1测量仪器 5
2.1.2测量技术 6
2.2样品溶液配制 7
2.3实验操作步骤 7
3.半边莲和半枝莲荧光光谱分析 8
3.1半边莲的荧光光谱特性 8
3.1.1半边莲的激发与发射光谱 8
3.1.2半边莲的偏振荧光光谱 9
3.2半枝莲的荧光光谱特性 11
3.2.1半枝莲的激发与发射光谱 11
3.2.2半枝莲的偏振荧光光谱 12
3.3半边莲和半枝莲荧光光谱对比 14
4.结论 15
致谢 16
参考文献 18
1绪论
中药是中华民族的传统精华，为中国传统中医特有药物，是我国悠久中医文化历经几千年沉淀，在救死扶伤医治伤病实践中逐渐形成的具有系统性科学性的药物。中药发源于中国，历史深厚，种类极多，分类上除了一些植物药以外，还有动物药，介壳类、矿物类等，只有少数中药来自于外国。
中药种植从古至今经过历朝历代的不断发展与传承，至今拥有了十分丰富的中草药资源,分布全国各地，除了物方面，我国还有着广泛的应用中草药治疗各类疾病的专业知识与人才基础。随着国家大力发扬中国传统文化，大力发掘中药产业，中药的用途越来越广泛，包括传统医治伤病、固本培元的作用，还包括结合西药优点提取特有物质创新发展中药产业。