而2-氯乙酰乙酸乙酯的沸点和反应产品4-氯乙酰乙酸乙酯相差很少（在减压条件下约为2-3℃），因此在精馏纯化时通常要反复蒸馏，造成产品的大量分解，最终导致收率偏低。
[1]沈开猷. 不饱和聚酯树脂及其应用．北京：化学工业出版社，1988
[2]甄谓先, 周菊兴. 制造纽扣的不饱和聚酯树脂. 北京师范大学学报（自然科学版）.1989，（1）：59-62
[3]黄量，戴立信. 手性药物的化学与生物学．北京：化学工业出版社，2002
[4]Rayond U L，Ulrike S．Advancesin Carbohydrate．Chem．And Biochem．1994，50：1
[5]Rouhi A．M．Chiral business．Z Chem Engineering,2003．5：45-55．
[6]刘燕，高哲石. 选择性5-羟色胺再摄取抑制剂的系统回顾与用药指南. 国外医药合成药生化药制剂分册. 2000，(21)：83-85
[7]褚忠义。生物合成药物学。化学工业出版社，2002．
[8]Bommarius A S，Riebel B R．Biocatalysis．WILEY-VCH Verlag GmbH．Co kGaA Weinheim USA，2004．
[9]David G．Morris．Stereochemistry．Royal SocietyofChem．，200 l．
[10]Rupley JA，Careri G．Protein hydration and function．Adv Protein．Chem．，1991，41：37—172．
[11]Kataoka M，Kita K Wada M，Yasohara Y，Hasegawa J，Shimizu S，Novel bioreduction system for the production of chiral alcohols，Appl Microbiol BiotechnoL 2003，62：437—445．
[12]Jean Canter，Alain Bergel，Maurice Comtat et a1．Coupling of the electroenzymatic
reduction ofNAD+with a synthesis reaction．Enzyme andMicrobial Technol，996，18：72-79．
[13]Miyashita A，Yasuda A, Takaya H, Totiumi K, Ito T, Souchi T, Noyori R. Synghesis of 2.2-bis（diphenylphosphino）-1.1-bionapthyl, and atropiomeric chiral bios （triaryl）phosphine, and its use in the rhodium（Ⅰ）-catalyzed asymmetric hydrogenation ofa-(acrylamino)acrylic acids. J.Chemi.Soci 1980,102;7932-7934.
[14]Kita K, Nakase K, Yanase H, Kataoka M,Shimisu S.Purification and characterization of new aldehyde reductases from Sporobolomyces salmonicolor AKU4429.J.Molecular Catalysis B;Enzymatic,1999,6:305-313.
[15]Kita K, KATAOKA M,SHIMIZU S.Diversity of 4-chloroacetoatate ethyl ester-reducing enzymes in yeasts and their application to chiral alcohol synthesis.J.Biosci and Bioengineering,1999,88:591-598.
[16]Kita K, Matsuzaki K,Hashimoto T,atal.Cloning of the Aldehyde Reductase Gene from a Red Yeast,Sporobolomyces salmonicolor, and Characterization of the Gene and Its Product,Appl.Environmental Microbiol,1996,52(7):2302-2310.
[17]Shimizu Sakayu,etc.Chiral alcohol synthesis with yeast carbonyl reductase.J.Molecular Catalysis B:Enzymatic,1998,5:321-325
[18]Shimizu Sakayu,etc.Stereoselective reduction of Ethyl 4-Chloro-3-oxobutanoate by a Microbial AIdehyde Reductase in an Organic Solvent-Water.Appl.Environ Microbiol,1990,8:2374-2377
[19]Song C E, Lee J K, Lee S H, Lee S. Tetrahedron: Asymmetry,1995,6(5):1063
[20]Kitamura M, Ohkuma T, Takaya H, Noyon R. Tetrahe-dron Lett,1988, 29(13):1555
[21]Marino J P,McClure M S, Holub D P, Comasseto J V, Tucci F C. J Am Chem Soc,2002,124(8)：1664
[22]Kolb H C, Bennani Y L, Sharpless K B. Tetrahedron: Asymmetry,1993,4(1):133
[23]Davies S G, Ichihara O. Tetrahedron: Asymmetry, 1996,7(7):1919
[24]Hikichi S, Hareau G P-J, Sato F. Tetrahedron Lett,1997,38(48):8299
[25]Zhou Y-G, Tang W, Wang W-B, Li W, Zhang X. J Am Chem Soc,2002, 124(18):4952
[26]Hikichi S, Hareau G P J, Sato F. Efficient Practical Synthesis of Optically Active 5-t-butyldimethylsiloxy-2-cyclohese-none as a convenient Chiral 2, 5-Cyclohexadienone Synthon. Tetrahedron Letters,1997,38:8299-8302
[27]Marino J P, McClure M S, Holib D-P, et al. Stereocentrolled Synthesis of (-)-Macrolactin A, J Am Chem Soc, 2002, 124(8): 1664-1668 4-氯乙酰乙酸乙酯文献综述和参考文献(2):http://www.751com.cn/wenxian/lunwen_62136.html