摘要：四环素类抗生素是目前使用最广泛、用量最大的抗生素种类之一, 其在环境中的大量残留带来了潜在的环境风险。本文介绍了四环素类抗生素的污染现状以及生态毒性。并以水华蓝藻铜绿微囊藻为研究对象，通过四环素对铜绿微囊藻的生长抑制、四环素对藻液pH值的影响、四环素对蛋白质含量的影响，研究四环素对蓝藻的急性毒性。结果表明：1 mg/L的四环素能够刺激铜绿微囊藻细胞的生长，而铜绿微囊藻在长时间暴露于高浓度四环素时其生长明显受到抑制。本文同时通过高效液相色谱法检测了四环素在铜绿微囊藻液中的降解情况，结果表明：该反应属于一级动力学反应，Ln（四环素浓度）与反应时间呈线性关系。为今后的研究方向进行了探计，旨在为其生态风险评价提供有价值的参考。
关键词： 四环素、铜绿微囊藻、急性毒性、抑制、降解4209
Acute toxicity of tetracycline on Microcystis aeruginosa
Abstract: Tetracycline antibiotics are widely used in the world. This is one of the largest types of antibiotics, which can remain in the environment and bring potential environmental risks. In this study, the eco-toxicity of tetracyclines on cyanobacteria Microcystis aeruginosa was studied. Growth inhibition of this kind of algae exposure to tetracycline was measured. pH values, protein content, were also studied to determine the acute toxicity of tetracycline on M. aeruginosa. The results showed that at low concentration 1 mg/L tetracycline can stimulate the growth of M. aeruginosa, and in prolonged exposure to high concentrations of tetracycline, M. aeruginosa growth was suppressed. This paper also detected the degradation of tetracycline in M. aeruginosa culture medium by high performance liquid chromatography (HPLC). The results showed that the reaction is a first-order kinetics reaction, Ln (tetracycline concentration) and the reaction time showed linear relationship. And discussed the future research directions, aimed to provide valuable reference for the ecological risk assessment of the antibiotics.
KeyWords: Tetracycline; Microcystis aeruginosa; Acute toxicity; Inhibit; Degradation
目录
1. 引言（绪论） 1
1.1 抗生素的污染现状 1
1.2 四环素类抗生素的污染现状 2
1.3 四环素类抗生素的生态毒性 3
1.3.1 四环素类抗生素对微生物的生态毒性 3
1.3.2 四环素类抗生素对植物的生态毒性 4
1.3.3 四环素类抗生素对动物的生态毒性 4
1.4 四环素的理化性质和药理毒理 5
1.4.1 四环素的理化性质 5
1.4.2 四环素的药理毒理 5
1.5 蓝藻、铜绿微囊藻 6
1.5.1 蓝藻概述 6
1.5.2 铜绿微囊藻概述 6
1.5.3 藻类水华 6
1.6 微囊藻毒素的污染现状 7
1.6.1 微囊藻毒素的污染现状 7
1.6.2 天然及水库水体的微囊藻毒素污染 7
1.6.3 饮用水的微囊藻毒素污染 8
1.7 课题研究的意义和目的 8
1.8 课题研究的内容 8
2. 实验条件及预实验 9
2.1 实验条件 9
2.1.1 实验仪器 9
2.1.2 实验材料 9
2.1.3 实验药品 11
2.2 预实验 11
2.2.1 BG11培养液的配置 11
2.2.2 高压蒸气灭菌 12
2.2.3 铜绿微囊藻的接种 12
2.2.4 铜绿微囊藻的培养 12
2.3 实验方案论证 12 四环素对蓝藻的急性毒性研究+实验讨论:http://www.751com.cn/yixue/lunwen_762.html