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摘要本文研究了具有吡啶降解能力的三株纯菌株Shinella granuli NJUST27，Paracoccus versutus NJUST28和Rhizobium sp. NJUST18的吡啶降解性能，并对这三株菌株在吡啶初始浓度范围100mg/L~1000mg/L内建立特效菌株的降解动力学模型，得出各降解菌株的动力学参数，并进行比较。实验结果表明，Haldane模型能够较好的拟合三株菌株的降解动力学曲线，得到的动力学常量为NJUST27：qSmax=0.245884g/g/h，Sm=719.7109mg/L，Ks=79.2mg/L，Ki=6540.2mg/L；NJUST28：qSmax=0.305545g/g/h，Sm=1087.162mg/L，Ks=71.5795mg/L，Ki=16512mg/L；由数据可知，两株菌株的最大比降解速率均高于NJUST18的qSmax=0.1212g/g/h；且比降解速率最大时的吡啶浓度也高于NJUST18的Sm=507.83mg/L，说明吡啶对菌的抑制作用小，菌能在较大吡啶浓度范围内生长。由此可知NJUST27、NJUST28的降解性能明显优于NJUST18。研究表明，建立吡啶特效菌的降解动力学模型可以对菌株降解吡啶的生化过程得到定量描述，具有较大的研究意义。27651
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关键词 吡啶 生物降解 Haldane模型 动力学
毕业论文设计说明书外文摘要
Title Study on the characterization of bacterium capable of degrading pyridine and its degradation kinetics
Abstract
This paper described the pyridine degradation performance of three pure strains with pyridine degradation ability, namely Shinella granuli NJUST27, Paracoccus versutus NJUST28 and Rhizobium sp. NJUST18. The degradation kinetics model of these three strains in the initial pyridine concentration range of 100mg/L~1000mg/L was established. Then the kinetic parameters of each strain could be obtained and compared. The results showed that the Haldane model could well fit the degradation kinetics curves of the three strains. The obtained kinetic constants were as follows: for NJUST27, qSmax=0.245884g/g/h, Sm=719.7109mg/L, Ks=79.2mg/L, Ki=6540.2mg/L; for NJUST28, qSmax=0.305545g/g/h, Sm=1087.162mg/L, Ks=71.5795mg/L, Ki=16512mg/L. The data shows that the maximum specific degradation rates of NJUST27 and NJUST28 were higher than that of NJUST18 (qSmax=0.1212 g/g/h). The pyridine concentrations at the maximum specific degradation rate (Sm) for NJUST27 and NJUST28 were higher than that of NJUST18, which was 507.83 mg/L. These results showed that the inhibition effect of pyridine on NJUST27 and NJUST28 was small, and these two strains could grow in a wide range of pyridine concentrations. From this, the degradation performance of NJUST28 and NJUST27 was significantly better than that of NJUST18. The results indicated that the biochemical process of the pyridine degrading strains could be quantitatively described by the degradation kinetics, which was of great significance for the study.
Keywords Pyridine Biodegradation Haldane model Kinetics
目 次
1 引言 1
1.1 吡啶的简介 1
1.2 吡啶类污染的处理方法 1
1.2.1 物理化学处理法 1
1.2.2 生化处理法 2
1.3 吡啶降解动力学研究 3
1.4 研究课题的提出和内容 3
1.4.1 研究课题的提出 3
1.4.2 研究内容 3
2 实验材料与方法 5
2.1 实验材料 5
2.1.1 实验药品 5
2.1.2 实验所需仪器 5
2.1.3 培养基组成 6
2.2 实验方法 6
2.2.1 菌种的保存 6
2.2.2 种子液的制备 6
2.2.3 菌的吡啶降解能力研究 6
2.2.4 动力学模型的建立 7
2.3 表征手段 8
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