摘 要:微生物引起的腐烂与品质劣变是枇杷采后贮藏过程中存在的主要问题,而二氧化氯(ClO2)被公认为一种安全、高效、“绿色”的杀菌剂。本试验研究了50、100、150mg/L ClO2结合0.8% CaCl2 处理(10min)对枇杷在非冷害低温(7.5±0.5) ℃和0.02 mm PE包装贮藏条件下抗氧化酶系统的变化情况,结果表明:与2个对照(清水CK1、0.8% CaCl2 CK2)、50 mg/L、150mg/L ClO2结合钙处理相比,100mg/L ClO2结合0.8% CaCl2复合处理能显著提高枇杷果实贮藏期间SOD、POD、CAT的活性,抑制MDA含量的积累,降低PPO活性,贮藏25d时果实外观和风品质良好。因此,将安全、高效的杀菌剂ClO2和生理调节剂CaCl2复合使用对枇杷果实能起到更好的保鲜效果。 54490
毕业论文关键词:枇杷;二氧化氯(ClO2);钙处理;抗氧化酶;贮藏保鲜
Effect of Chlorine Dioxide Combined with Calcium Treatment on Loquat Antioxidant Enzyme Activity
Abstract:The decay and quality deterioration caused by microorganism is the main problems existing in the process of loquat postharvest storage,and the chlorine dioxide (ClO2) is recognized to be a safe,efficient,green bactericide.This experiment studied the change of antioxidant enzyme system of loquat fruit with the 50,100,150 mg/L ClO2 combined with 0.8% CaCl2 treatments for 10 min were assessed under the condition of non-chilling low temperature(7.5 + 0.5) ℃ and 0.02 mm PE packaging and storage.The experimental results showed that compared with the two control (water CK1, 0.8% CaCl2 CK2),50,150 mg/L ClO2 combined with calcium treatment,100 mg/L ClO2 combined with 0.8% CaCl2 composite treatment can significantly improve the activity of SOD,POD,CAT during the storage of loquat fruits,and inhibit the accumulation of MDA content,reduce the activity of PPO,25 d storage fruit appearance and flavor quality is best,therefore,the safe,effective,cheap fungicide ClO2 and the physiological regulator CaCl2 composite used for loquat fruit can have better effect.
Keywords: Loquat, Chlorine dioxide (ClO2), Calcium treatment, Antioxidant enzymes, Storage and fresh-keeping
目 录
前言 4
1 材料与方法 4
1.1 试验材料 4
1.2 试验设计与处理 4
1.3 检测指标与方法 5
1.3.1 超氧化物歧化酶(SOD)活性的测定 5
1.3.2 过氧化物活性酶(POD)活性的测定 5
1.3.3 过氧化氢活性酶(CAT)活性的测定 5
1.3.4 丙二醛(MDA)含量的测定 5
1.3.5 多酚氧化酶(PPO)活性的测定 6
2 结果与分析 6
2.1 不同浓度ClO2结合钙处理对枇杷SOD活性的影响 6
2.2 不同浓度ClO2结合钙处理对枇杷CAT活性的影响 6
2.3 不同浓度ClO2结合钙处理对枇杷过氧化物酶活性的影响 7
2.4 不同浓度ClO2结合钙处理对枇杷丙二醛含量的影响 7
2.5 不同浓度ClO2结合钙处理对枇杷多酚氧化酶活性的影响 8
3 讨论 8
结 论 10
参考文献 11
致 谢 13
前言
枇杷起源于我国四川雅安地区,果实甜酸爽口多汁,具有丰富的营养和清肺、止咳等药用价值。在江苏地区,枇杷成熟于6月初前后期,采后常温下贮藏1周极易发生腐烂、褐化和衰老变质,导致供应期短和经济损失。微生物引起的腐烂是枇杷果实品质下降和保鲜期短的主因之一[1],1-5℃低温贮藏虽能抑制果实腐烂的发生,但会出现果肉褐变、出汁率减少等冷害不良症状[2],因此在非冷害低温贮藏条件下探寻安全、有效、经济的防腐保鲜剂仍是解决枇杷等果蔬贮藏保鲜问题的关键技术之一。