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摘要：土霉素是目前在全球应用最为广泛并且拥有一定产量的抗生素，残留于环境中对生态环境有着严重的生态风险。藻类水华现象是常见于湖泊中的又一水生生态问题，而藻毒素是伴随着藻类水华出现的一个严重问题。作为共存于水生环境中的两个危害水生生态系统的要素，两者之间存在何种联系值得我们探究。本毕业论文主要研究土霉素对于铜绿微囊藻生长情况的作用效果以及对铜绿微囊藻产生和释放藻毒素的影响，本实验数据表明短期内在0.2mg/L浓度土霉素下的铜绿微囊藻生长较对照组长势更快，是一个危险的生态值。在藻毒素的生成与释放研究中，1mg/L浓度的土霉素是刺激藻毒素释放的浓度值，而5mg/L浓度的土霉素则能最大程度抑制藻毒素生成释放。本实验的研究为我们通过控制土霉素浓度从而抑制铜绿微囊藻的大量生长提供一定的理论基础，为高浓度土霉素作为藻毒素抑制剂的使用做了初步的探究。20105
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关键词：土霉素；铜绿微囊藻；藻毒素；抑制
The research of Oxytetracycline effect on the release of Microcystis aeruginosa microcystin at ambient levels
Abstract: oxytetracycline is one of antibiotics which currently the most widely used in the world and have a steady output，it residues in the environment has serious ecological risks for the ecological environment.Algal Blooms is a another common aquatic ecosystems problem in lakes， and microcystin was the serious problem coming with the algal blooms.As two hazardous factor to the aquatic ecosystem co-exist in the aquatic environment，there is contact between the two which is worth exploring.In this paper，the main research is about under the effect of oxytetracycline ，the change of the Microcystis aeruginosa growth condition and production and release of microcystin，The experimental data show that the Microcystis aeruginosa under the effect of 0.2mg/L oxytetracycline grow faster than the control group in short time.0.2mg/L oxytetracycline is a dangerous ecological value.In the production and release of microcystin research ，1mg/L concentration of oxytetracycline stimulate released of microcystin and 5mg/L concentration of oxytetracycline can make the maximum inhibiting to the production and release of microcystin concentration.After this research ，it provide us a theoretical basis on control of the concentration of oxytetracycline to inhibit the growth of Microcystis aeruginosa ，make a primary research for the high concentration of oxytetracycline used as a microcystin inhibitor.
Keywords:Oxytetracycline， Microcystis aeruginosa， Microcystin， Suppression
目录
1. 引言（绪论） 1
1.1 抗生素的污染现状 1
1.1.1概况 1
1.1.2土霉素（四环类抗生素）的污染现状 1
1.1.3水环境中抗生素污染 2
1.2 土霉素（四环类抗生素）的生态毒性 3
1.3 蓝藻概况 3
1.3.1 蓝藻 3
1.3.2 铜绿微囊藻 4
1.3.3 藻类水华与水体富营养 4
1.3.4微囊藻毒素毒性 4
1.3.5微囊藻毒素的产生 5
1.3.6微囊藻毒素污染现状 6
1.4 课题研究的意义和目的 7
1.5 课题研究内容 8
2. 实验条件 9
2.1 实验药品 9
2.2实验仪器 9
2.3实验材料 10
3. 实验方法与内容 12
3.1 预实验 12
3.1.1 BG11培养液的配置 12
3.1.2 高压蒸气灭菌 12
3.1.3 铜绿微囊藻的接种 13