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摘要：本试验旨在研究DL-蛋氨酸对宫内发育迟缓（IUGR）哺乳仔猪肠道发育及抗氧化功能的影响。试验在新生仔猪中选择日龄相同的10头IUGR仔猪和5头正常初生重（NBW）仔猪，分为3个处理组（n=5/组）。其中5头NBW仔猪饲喂基础人工乳，10头IUGR仔猪一半饲喂基础人工乳、一半饲喂基础人工乳+0.12% DL-蛋氨酸。结果显示：与NBW哺乳仔猪相比，IUGR哺乳仔猪回肠绒毛高度、绒毛高度/隐窝深度显著降低（P < 0.05），空肠黏膜乳糖、蔗糖酶酶活性显著降低（P < 0.05）。另外，IUGR显著降低哺乳仔猪空肠黏膜还原型谷胱甘肽（GSH）含量、还原型谷胱甘肽/氧化型谷胱甘肽比值（P < 0.05）和回肠GSH含量（P < 0.05），显著升高空肠黏膜蛋白质羰基（PC）含量和丙二醛含量（P < 0.05）。添加DL-蛋氨酸可显著提高IUGR哺乳仔猪空肠和回肠的绒毛高度、回肠绒毛高度/隐窝深度的比值（P < 0.05）以及空肠蔗糖酶酶活性（P < 0.05），同时使空肠GSH含量显著升高（P < 0.05）、PC含量显著降低（P < 0.05）。因此，添加DL-蛋氨酸可在一定程度上缓解IUGR引发的氧化损伤，对IUGR哺乳仔猪肠道发育具有改善作用。25774
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毕业论文关键词：DL-蛋氨酸；宫内发育迟缓；哺乳仔猪；肠道发育；抗氧化功能
Effects of DL-Methionine on intestinal development in intrauterine growth-retarded suckling piglets
Abstract: The present study was designed to evaluate the effects of DL-Methionine (DL-Met) on intestinal development in intrauterine growth-retarded (IUGR) suckling piglets. Five normal birth weight (NBW) and ten IUGR newborn piglets were selected and assigned to the following 3 experimental groups (n=5/group). Five normal birth weight piglets were fed a basic formula milk and the other 10 IUGR piglets were fed either a basic formula milk or a basic formula milk with + 0.12% DL-Met. The results showed that: Compared with NBW piglets, IUGR piglets had lower villus height and villus height/crypt depth ratio in ileum (P < 0.05). There were significant decreases in activities of lactase and sucrase in jejunum (P < 0.05). In addition, IUGR piglets had decreased glutathione (GSH) content and glutathione/glutathione ratio, whereas increased malondialdehyde (MDA) and protein carbonyl (PC) in jejunum (P < 0.05). Furthermore, the content of GSH was also significantly decreased in the ileum of IUGR piglets (P < 0.05). However, DL-Met treatment increased the villus height in both jejunum and ileum, and the villus height/crypt depth ratio in ileum (P < 0.05). DL-Met alleviated the decreased activity of sucrase in the jejunum of IUGR-MET piglets (P < 0.05). DL-Met supplementation significantly increased the GSH content, whereas decreased the PC content in jejunum (P < 0.05). These results indicated that DL-Met can alleviate the oxidative damage caused by IUGR and improve the intestinal development of IUGR suckling piglets.
Key words: DL-Methionine; Intrauterine growth-retardation; Suckling piglets; Intestinal development; Antioxidant function
目 录
摘要 1
关键词 1
Abstract 1
Key words 1
引言 2
1 材料与方法 2
1.1 试验设计 2
1.2 样品采集 3
1.3 测定指标及方法 3
1.3.1 肠道组织形态变化的观察 3
1.3.2 肠道黏膜中二糖酶酶活性以及抗氧化功能的检测 3
1.3.3 肠道组织目的基因mRNA表达量的测定 3
1.4 数据统计与分析 4
2结果与分析 4
2.1 DL-蛋氨酸对IUGR哺乳仔猪肠道组织形态的影响 4