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摘要：花青苷是一种水溶性色素，广泛存在于植物的体内，是非常有潜力的天然色素的来源。因资源丰富、颜色鲜艳、无毒安全和具有抗氧化性等优势，花青苷的研究和开发利用备受关注。6-BA是一种人工合成的细胞分裂素，不但在细胞生长和分化及其他相关的生理活动过程中起重要作用，还可以促进花青苷的积累，抑制或促进相关酶的活性。本实验以不结球白菜紫色品种NJX1-4和绿色品种苏州青为材料，利用外源6-BA处理植株，并测定不同时间点叶片中花青苷含量，抗氧化性及相关酶活性，探索6-BA对不结球白菜中花青苷的抗氧化性及相关酶活性的调控作用，为今后进一步研究花青苷的功能机制提供理论基础。26233
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毕业论文关键词：花青苷；6-BA；抗氧化性；酶活性，不结球白菜
Effect of exogenous 6-BA on anthocyanins antioxidant activity and related enzyme activities in Purple non-heading Chinese Cabbage
Student majoring in Protected agriculture science and engineering Chula Sa
Tutor Chunmei Hu
Abstract： Anthocyanins are water-soluble pigments which are widely found in plants, it is a potential source of natural pigments. The research and development of anthocyanins are important for its rich resources, bright colors, safe and antioxidant activity.6-BA is a synthetic cytokinin. It plays an important role in the cell growth and differentiation and other related physiological activities of the process. In addition, 6-BA can promote the accumulation of anthocyanins, as well as inhibit or induce other enzyme activity. In this study, we used two self-line of non-heading Chinese Cabbage: purple leaves NJX1-4 and green suzhouqing as the experimental materials. Leaves were treaded by exogenous 6-BA to explored the effect on anthocyanins antioxidant activity and related enzyme activities, providing a theoretical basis for further study of the functional mechanism of anthocyanins.
Key words: Anthocyanins; 6-BA; Antioxidant activity; Enzyme activity; non-heading Chinese Cabbage
目 录
摘要 3
关键词 3
Abstract 3
Key words 3
1 材料与方法 4
1．1 试验材料 5
1．2 试验方法 5
1．2．1 外源6-BA处理 5
1．2．2 总花青苷含量测定 5
1．2．3 总酚含量测定 5
1．2．4 抗氧化酶活性测定 5
1．2．5 叶片花青苷的抗氧化活性分析 6
1．3 数据分析 6
2 结果与分析 6
2．1 外源6-BA对不结球白菜中花青苷含量的影响 6
2．2 外源6-BA对不结球白菜叶片中总酚含量的影响 7
2．3 外源6-BA对不结球白菜叶片中相关酶活性的影响 8
2．3．1 外源6-BA处理对紫色不结球白菜中超氧化物歧化酶（SOD）活性的影响 8
2．3．2 外源6-BA处理对紫色不结球白菜中过氧化物酶（POD）活性的影响 8
2．3．3 源6-BA处理对紫色不结球白菜中过氧化氢酶（CAT）活性的影响 9
2．4 叶片抗氧化活性测定 9
3 讨论 10
3．1 外源6-BA处理不结球白菜后总花青苷含量和总酚及抗氧化能力的比较 10
3．2 外源6-BA处理对不结球紫色白菜中抗氧化酶的影响 10
3．3 外源6-BA处理对不结球紫色白菜中花青苷的抗氧化性影响 11