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摘要：硫化氢(H2S)是一种新发现的气体信号分子，近年来的研究表明它可以增强植物耐盐胁迫的能力，但尚不明确其具体的作用机制。针对上述问题，本研究以CP3508、J4375及其杂交子代F1的油菜幼苗为材料。结果表明：在100 mM 氯化钠溶液盐胁迫下亲本和杂交后代中硫化氢含量上升；亲本L-半胱氨酸脱巯基酶活性均上升，而子代中活性变化不明显；两种亲本丙二醛(Malondialdehyde,MDA)和脯氨酸含量也呈现上升趋势，杂交后代F1则几乎不受影响。在该项研究中硫化氢含量的变化和L-半胱氨酸脱巯基酶活性关联不大，特别是在杂交后代F1受到100 mM氯化钠盐胁迫时该酶活性不变却伴随着硫化氢含量的上升，意着在杂交后代F1中有着其他产生硫化氢的机制。该研究为探究新的内源性硫化氢代谢机制提供理论依据，并为提高油菜产量提供新的思路。26678
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毕业论文关键词：硫化氢；L-半胱氨酸脱巯基酶；盐胁迫；油菜
A Primary Research toward the Effects of Salinity Stress on Endogenous Hydrogen Sulfide Metabolism and Oxidative Damage in Two Breeds of Rapeseeds and Their Hybrid Progeny
Abstract: Hydrogen sulfide (H2S) is a newly discovered gas signal molecule. Recent studies have shown that it can enhance the ability of plant salinity tolerance, but its specific mechanism is not clear. To solve this problem, two breeds of rapeseeds: CP3508, J4375 (Brassica campestris L., CP3508, J4375) and their hybrid F1 was used as materials. The results showed that the content of hydrogen sulfide increased in parents and hybrid F1 under the salinity stress of 100 mM sodium chloride (NaCl) solution; the activities of L-cysteine desulfase (L-CDes) of parents were increased when they were in 100 mM NaCl stress, but change in the activity of this enzyme is not significant; the contents of malondialdehyde (MDA) and proline were also increased in parents, while F1 was almost unaffected in this case. In this study, the change in hydrogen sulfide content was not associated with the change in L-CDes activity, especially when the hybrid F1 were treated with 100 mM NaCl stress; the increase in hydrogen sulfide content also showed that there were other mechanisms to produce hydrogen sulfide in the hybrid F1. This study provides a theoretical basis for exploring the new endogenous hydrogen sulfide metabolism mechanism and provides new ideas for improving rape yield.
Key words: Hydrogen sulfide (H2S); L-cysteine desulfase (L-CDes); salinity stress; Rapeseed (Brassica campestris L.)
目 录
摘要1
关键词1
Abstract1
Key Words..1
1引言2
2材料与方法2
2.1材料2
2.2样本采集2
2.3 实验方法3
2.4 统计分析...3
3 结果和分析..3
3.1盐胁迫诱导内源性硫化氢含量提升4
3.2盐胁迫诱导亲本中L-半胱氨酸脱巯基酶总酶活提升5
3.3亲本幼苗在盐胁迫下脯氨酸含量有明显提升6
3.4盐胁迫条件会对亲本和杂交子代中丙二醛含量产生不同结果7
4 结论..8
4.1盐胁迫会对亲本造成氧化损伤并提高丙二醛和脯氨酸含量8
4.2杂交后代F1受到盐胁迫诱导的氧化损伤较少8
4.3内源性硫化氢的产生可能是植物抗盐胁迫的机制之一8
4.4 讨论8
致谢9
参考文献10
盐胁迫对两种油菜品种及其杂交后代的内源性硫化氢代谢和氧化损伤影响的初步研究1. 引言:
我国是一个土地资源丰富但人均稀少的国家，根据十年前的官方调查结果，中国的可耕地面积为18.24亿亩[1]，位居世界第三且需要供给世界上第一人口大国所需的农副产品，而在中国珍贵的可耕地中，有着很大一部分受到盐碱化危害。盐胁迫会给植物带来多种损伤，而其中由盐胁迫诱导的氧化损伤被认为是盐胁迫的机制之一。氧化损伤是植物体内氧自由基的产生和清除之间的平衡被打破，产生的氧自由基大于被清除的氧自由基，多余的氧自由基会破坏细胞膜结构和生物分子，对植物产生损伤[2]。氧自由基破坏生物膜中的脂质会产生丙二醛，测定丙二醛含量是判断植物受到氧化损伤程度的重要方法之一。脯氨酸是植物受到氧化损伤产生的物质之一，脯氨酸含量的提高往往意着受到更严重的氧化损伤。