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摘要：[目的] 探讨汞的96h的半致死浓度和安全浓度对泥鳅的体内抗氧化系统的影响，及其肝胰脏、鳃、卵巢和脑组织中抗氧化酶系活性及MDA含量的变化特征。[方法] 将48条泥鳅分为汞离子的半致死浓度组、安全浓度组、空白组，半致死浓度组的汞的浓度是0.26 mg/L、安全浓度组的汞浓度是0.026 mg/L、空白组不加重金属汞。在实验室染毒96h后将活着的泥鳅进行解剖，取肝胰脏、鳃、卵巢、脑备用。 [结果] 96h汞的半致死浓度组泥鳅肝胰脏的T-AOC活性显著降低，MDA含量显著上升；而卵巢的T-AOC活性显著降低，MDA含量显著降低；鳃的T-AOC活性显著上升，MDA含量显著下降；脑的T-AOC活性显著上升，MDA含量显著下降。[结论] 汞可引起泥鳅卵巢组织和肝胰脏T-AOC活力、MDA含量改变, 表明汞可引起体内氧应激反应导致脂质过氧化损伤。31560
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毕业论文关键词：半致死浓度；泥鳅；汞；总抗氧化能力（T-AOC）；丙二醛（MDA）
Effects of Mercury on the Antioxidant Ability of Loach
Abstract: [Objective] to investigate the effects of mercury 96h half lethal concentration and the safe concentration of loach of antioxidant system, characteristics and hepatopancreas, gills, ovary and brain tissue T-AOC activity and MDA content. [Methods] 48 loach as mercury ion concentration, semi lethal concentration group security group, blank control group, the concentration of mercury in the semi lethal concentration group is mercury concentration is 0.026mg/L 0.26mg/L, the security group, blank group without heavy metal mercury. In the laboratory after 96h exposure will live loach were dissected, hepatopancreas, gill, ovary and brain, spare. [results] 96h mercury semi lethal concentration of Loach Liver and pancreas of group T-AOC activity was significantly decreased, MDA content increased significantly; and the ovarian T-AOC activity decreased significantly, decreased the content of MDA increased significantly with; the activity of T-AOC, MDA content decreased significantly; the brain activity of T-AOC significantly increased, MDA content decreased significantly. [Conclusion] mercury can cause the ovary and hepatopancreas of Misgurnus anguillicaudatus T-AOC activity, MDA content change, indicated that mercury can cause oxidative stress leading to lipid peroxidation injury reaction in vivo.
Keywords: semi lethal concentration; loach; mercury; total antioxidant capacity(T-AOC); malondialdehyde (MDA)
目录
引言 1
1 材料与方法 2
1.1 主要试剂 2
1.2 实验动物 2
1.3 主要仪器 2
1.4 方法 2
1.4.1饲养条件 2
1.4.2染毒 2
1.4.3 酶样的制备 3
1.4.4 总抗氧化能力和MDA含量测定方法 3
1.4.5 统计分析方法 3
2 结果与分析 3
2.1 泥鳅0.26 mg/L组、0.026 mg/L组与空白组相比肝胰脏总抗氧化能力变化 4
2.2泥鳅0.26 mg/L组、0.026 mg/L组与空白组相比卵巢总抗氧化能力变化 4
2.3泥鳅0.26 mg/L组、0.026 mg/L组与空白组相比鳃总抗氧化能力变化 5
2.4泥鳅0.26 mg/L组、0.026 mg/L组与空白组相比脑总抗氧化能力变化 5
2.5泥鳅0.26 mg/L组、0.026 mg/L组与空白组相比肝胰脏MDA含量变化 6
2.6泥鳅0.26 mg/L组、0.026 mg/L组与空白组相比卵巢MDA含量变化 7
2.7泥鳅0.26 mg/L组、0.026 mg/L组与空白组相比鳃MDA含量变化 7
2.8泥鳅0.26 mg/L组、0.026 mg/L组与空白组相比脑MDA含量变化 8
3 讨论 9
致谢： 11
参考文献 12