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摘要：采用不同浓度的人工污水和复合菌肥浇灌盆栽玉米，研究复合菌肥对污灌玉米土壤酶活性的影响。结果表明：随污水浓度的升高，土壤蔗糖酶，淀粉酶，脲酶，蛋白酶，过氧化氢酶活性均呈现先升高后下降的趋势，土壤蔗糖酶，淀粉酶在污水浓度为50%时酶活性达到最大，分别为0.37mg葡萄糖/g土壤、0.07mg麦芽糖/g土壤；土壤脲酶，蛋白酶在污水浓度为25%时酶活性达到最大，分别为0.21mgNH3-N/g土壤、1.43mg氨基氮/g土壤；土壤过氧化氢酶在污水浓度为75%时酶活性达到最大，为0.65mL (0.1mol /LKMnO4)/(h•g)。采用复合菌肥浇灌玉米后，土壤蛋白酶活性变化不规律，而土壤蔗糖酶、淀粉酶、脲酶、过氧化氢酶活性均有所提高，土壤蔗糖酶、淀粉酶、脲酶活性随复合菌肥浓度的升高而增强，土壤过氧化氢酶活性变化不规律。5785
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关键词：复合菌肥；土壤酶；污灌；酶活性
Effects of Combined Bacterial Manure on Soil Enzyme Activities in the Soil of Sewage Irrigation Corn
Abstract: The different concentrations of synthetic wastewater and combined bacterial manure were used to irrigate the potted corn. Effects of combined bacterial manure on soil enzyme activities in the soil of sewage irrigation corn were studied. The results showed that the enzyme activities of invertase, amylase, protease, urease, catalase presented the law of first increasing and then decreasing along with the sewage concentration increasing.The enzyme activities of invertase and amylase were the highest, 0.37mg glucose/g soil, 0.07mg glucose maltose/g soil respectively at concentration of sewage of 50%. The enzyme activities of urease and protease were the highest, 1.43mg amino nitrogen/g soil, 0.21mgNH3-N/g soil respectively at concentration of sewage of 25%. The enzyme activities of catalase were the highest, 0.65mL (0.1mol/LKMnO4) /(h•g) at concentration of sewage of 75%. Soil protease activity changed irregularly, and soil invertase, amylase, urease,catalase activities presented the law of increasing after irrigating the different concentrations of photosynthetic bacteria. Soil invertase, amylase, urease activities presented the law of increasing along with the concentration of photosynthetic bacteria increasing, but soil catalase changed irregularly.
Key words: Multiple bacteria fertilizer; Soil enzymes; Sewage irrigation; Enzymatic activity
目 录
摘 要 1
Abstract.1
引言 2
1 材料与方法 2
1.1 材料 2
1.2 试验设置与处理 3
1.3 仪器和设备 3
1.4 土壤酶活性测定 3
1.4.1 土壤蔗糖酶活性的测定 4
1.4.2 土壤淀粉酶活性的测定 4
1.4.3 土壤脲酶活性的测定 5
1.4.4 土壤蛋白酶活性的测定 6
1.4.5 土壤过氧化氢酶活性的测定 7
1.5 数据处理 7
2 结果与分析 8
2.1 污水灌溉和复合菌肥对土壤蔗糖酶活性的影响 8
2.1.1 土壤蔗糖酶标准曲线 8
2.1.2 不同浓度的污水灌溉和不同浓度的复合菌肥对土壤蔗糖酶活性的影响 8
2.2 污水灌溉和复合菌肥对土壤淀粉酶活性的影响 9
2.2.1 土壤淀粉酶标准曲线 9
2.2.2 不同浓度的污水灌溉和不同浓度的复合菌肥对土壤淀粉酶活性的影响 9
2.3 污水灌溉和复合菌肥对土壤脲酶活性的影响 10
2.3.1 土壤脲酶标准曲线 10
2.3.2 不同浓度的污水灌溉和不同浓度的复合菌肥对土壤脲酶活性的影响 10