摘要:采用水培实验方法,研究了铈对紫外辐射胁迫下油菜幼苗叶片中叶绿素含量的影响。结果表明:紫外辐射胁迫会导致油菜幼苗叶片中叶绿素总量降低,并且叶绿素a的下降幅度大于叶绿素b。适宜浓度的氯化铈(本实验条件下最适浓度为20 mg/L)有提高油菜幼苗叶绿素总量和叶绿素a/叶绿素b值的作用,较高浓度的铈则抑制油菜幼苗叶片中叶绿素的合成,并且对叶绿素a的影响大于叶绿素b。这说明合适浓度的铈溶液能缓解紫外辐射胁迫对油菜幼苗光合器官结构的破坏,增强油菜幼苗的抵抗能力。43016
毕业论文关键词:油菜;铈;紫外辐射胁迫;叶绿素
Effects of Cerium on Chlorophyll Content of Rape Seedlings
under UV Radiation Stress
Abstract: Adopting hydroponic experimental methods to study the effects of cerium on chlorophyll content of rape seedling leaves under ultraviolet radiation stress. The results showed that total chlorophyll of rape seedling leaves was reduced under ultraviolet radiation stress and falling range of chlorophyll a was greater than chlorophyll b. Suitable concentration of cerium chloride (optimal concentration of cerium chloride was 20 mg/L in this experimental conditions) could improve total chlorophyll and chlorophyll a / chlorophyll b value of rape seedling, while higher concentration of cerium chloride could inhibit synthesis of chlorophyll of rape seedlings leaves and had greater effect on chlorophyll a than chlorophyll b. All above showed that suitable concentration of cerium solution could alleviate UV radiation damage to the structure of rape seedling photosynthetic organs and enhance resistance of rape seedling.
Key Words: Rape; Cerium; Ultraviolet radiation stress; Chlorophyll
目 录
摘 要 1
引言 2
1. 材料与方法 2
1.1 实验材料 2
1.2 试剂 2
1.3 实验方法 2
1.3.1氯化铈溶液的配制 3
1.3.2油菜种子的消毒、浸泡 3
1.3.3油菜幼苗的培养及实验处理 3
1.3.4叶绿素含量的测定 3
2. 结果与分析 4
2.1氯化铈喷洒浓度对油菜幼苗叶片中叶绿素含量的影响 4
2.2氯化铈喷洒浓度对紫外辐射胁迫下油菜幼苗叶片中叶绿素含量的影响 4
3. 结论与讨论 6
3.1 结论 6
3.2 讨论 6
参考文献 7
附录 10
致谢 14
铈对紫外辐射胁迫下油菜幼苗叶绿素含量的影响引言
油菜又常常被人们称为苦菜,油白菜,是十字花科一年生草本植物,它的故乡在中国,油菜茎的颜色是深绿色的,但是油菜花的花骨朵却是黄色的,在农园艺学中通常将植物中种子含油的多个物种称之为油菜。油菜大多生长在气候湿润的地方,如中国的南方。在我国,油菜主要分布在山东、江苏、四川、陕西、安徽等地。油菜的营养很丰富,因为它含有多种大量的维生素,有维生素A、维生素C、维生素D和维生素E,是人体内脂溶性维生素的比较重要的来源。油菜有很多用途,幼嫩的油菜花可以供人们食用,油菜花败谢后,它的油菜籽又可以榨油。油菜籽榨出的油除了供人们食用外,在工业方面也有其一定的利用价值,如润滑油与脱膜剂,燃料油,淬火油等。因为油菜在人们日常生活中扮演着重要角色,因此研究油菜的抗逆性,有着重要的意义。