摘要厌氧氨氧化菌的保存在厌氧氨氧化工艺的应用研究中是一个关键问题。不同的低温保护剂,二甲基亚砜(DMSO)和海藻糖被用于在4 ℃和-40 ℃的贮藏温度下去构建一个保藏体系。厌氧氨氧化颗粒污泥经过两个月的保藏,反应器的性能和颗粒污泥活性恢复及颗粒特性变化试验揭示了不同保藏方法的优点和缺点。最后发现,选取4 ℃为保藏温度并未添加低温保护剂的保藏体系最优。经最优保藏体系保藏颗粒污泥后,在恢复期间,总氮去除率达到4.41 kg·m-3·d-1。在添加低温保护剂的试验中,DMSO是一种较优的低温保护剂,而海藻糖则有着惊人和意想不到的作用效果。除了在保藏期间污泥暴露于海藻糖的两个反应器外,修饰的Stover-Kincannon模型适用于描述存储后厌氧氨氧化颗粒污泥反应器恢复时的脱氮性能。47523
Abstract The preservation of anaerobic ammonium oxidation (anammox) bacteria is a key element in the study and application of the anammox process. Different cryoprotectants (dimethyl sulfoxide and trehalose) at storage temperatures of 4 °C and -40 °C were selected to construct a preservation system. After two months of storage, the performance of the reactors and the particle characteristics from the activity recovery test revealed the advantages and disadvantages of the different storage methods. Ultimately, the preservation method adopted was storage without cryoprotectant at 4 °C. With the aid of this optimized method, the nitrogen removal rate reached 4.41 kg·m-3·d-1 during the recovery period. Among the cryoprotectants assessed, dimethyl sulfoxide was found to be an appropriate cryoprotectant, and trehalose had surprising and unexpected effects. The modified Stover-Kincannon model was suitable for describing the nitrogen removal performance of the reactors with sludge after storage, except for the two reactors in which the granules were exposed to trehalose during the storage period.
毕业论文关键词:厌氧氨氧化菌;颗粒污泥;低温贮藏;活性恢复; 低温保护剂
Keyword: anammox bacteria; biogranule; cryopreservation; recovery activity; cryoprotectant
目 录
1 引言 3
2 材料和方法 3
2.1 接种物 3
2.2 储藏体系 4
2.3 反应器恢复过程 4
2.4 分析测定方法 4
2.5 Stover-Kincannon 模型 5
3 结果与讨论 5
3.1 厌氧氨氧化颗粒污泥的生物学特性 5
3.1.1 颗粒污泥活性 5
3.1.2 反应器的性能 6
3.1.3 动力学分析 8
3.1.4 化学计量比 9
3.2 厌氧氨氧化颗粒污泥特性 10
3.2.1 沉降速度 10
3.2.2 胞外聚合物 11
3.2.3 血红素含量 11
4 结论 12
参考文献 13
致谢 16
1 引言
厌氧氨氧化(anammox)是一个利用NH4+作为电子供体和NO2-作为电子受体的微生物反应[1]。厌氧氨氧化工艺的应用可降低成本、节约能源、提高处理效率,因而引起学者广泛关注。厌氧氨氧化菌的培育是厌氧氨氧化工艺运行的唯一原料来源。在上世纪90年代厌氧氨氧化菌被应用于中试规模的反硝化系统之后,其研究从理论研究转换到实际应用中[2,3]。此外,厌氧氨氧化菌对于海洋生物脱氮的贡献约占总数的50 %[4]。