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摘要此次实验的宗旨是研究验证ACTA1（α－肌动蛋白1）基因在徐淮山羊（胎羊）不同组织（心、肝、脾、肺、肾、肠、背肌、腿肌）中的表达差异，利用的手段是使用实时荧光定量PCR的技术。这为了以后研究ACTA1基因在有关胎羊的徐淮山羊上的问题打下基础。实验操作的步骤是：第一，采取徐淮山羊胎羊的心、肝、脾、肺、肾、肠、背肌、腿肌等部位的组织若干份；第二，提取八个组织总的RNA，并测量与记录RNA的浓度；第三，利用ACTA1基因特异性引物将各个部分组织的RNA逆转录成cDNA；最后使用PCR技术来检测其表达谱。实验最终结果表明：ACTA1基因在八个组织内均有表达，但存在表达差异。其表达量由高到低依次为背肌（13.177070）>腿肌（6.431454）>心（1.00000）>肝(0.466959)>肾(0.381187>肺(0.118584)>脾(0.086988)>肠(0.071708)。用心肌作为参照，ACTA1基因在背肌和腿肌中的表达量是极显著的，在脾、肺和肠中的表达量是显著，在肝和肾中表达不显著。此结果可以作为今后对徐淮山羊（胎羊）ACTA1基因研究的实验基础和理论依据。51005
该论文有图5幅，表4个，参考文献17篇。
毕业论文关键词：ACTA1基因 徐淮山羊 表达量
The study on different expression of ACTA1 gene in fetal lamb organs of Xuhuai goat
Abstract
The aim of the experiment research validation ACTA1 actin 1 (alpha) in different organization Xuhuai goats (fetal lamb) (heart, liver, spleen, lung, kidney, intestine, back muscle, leg muscle) expression in the difference, using the method is to use PCR technology.In order to investigate the ACTA1 gene in question on the adult Xu Huai goats on the basis. Experimental operation steps are: first, take the adult Xu Huai goat heart, liver, spleen, lung, kidney, intestine, back, leg muscle and so on several parts of the organization; second, the total RNA extraction of eight organization, and to measure and record the concentration of RNA; third, using ACTA1 gene specific primers to various parts of the organization's RNA reverse transcription into cDNA; finally using PCR technique to detect the expression profiles . The experimental results show that ACTA1 genes are expressed in eight in the organization are, but to express differences. The expression quantity order from high to low is back muscle (13.177070) > leg muscle (6.431454) > heart muscle (1.000000)> liver (0.466959)> kidney (0.381187) > lung (0.118584) > spleen (0.086988) > intestine (0.071708). With myocardial as a reference, ACTA1 amount of gene expression in back and leg muscle is very significant, the amount of expression in the spleen, lungs and intestine is significant, no significant expression in the liver and kidney. Experimental results can be used as the future of Xuhuai goats (fetal lamb) ACTA1 gene research experimental foundation and theoretical basis.
Key words: ACTA1 gene Xuhuai goat expression
目 录
摘要 I
Abstract II
目 录 III
图清单 IV
表清单 IIV
1引言 1
2材料与步骤 2
2.1实验材料 2
2.2 实验流程 3
3实验分析 5
3.1 定量分析 5
3.2 PCR数据处理 8
3.3 ACTA1在羊不同组织中的表达量 8
4.讨论