- 上一篇：聚季铵盐PDM对假单胞菌的杀生作用及机理分析初探
- 下一篇：生物分子的高灵敏荧光检测
摘要本文以耐甲氧西林金黄色葡萄球菌（MRSA）为实验对象，对实验室的2种药用植物中的提取部位及单体化合物进行了抑制和破坏MRSA生物被膜的研究。具体是在96-孔微量培养板中做天然单体化合物对MRSA生物被膜形成抑制实验，结晶紫染色来定量生物被膜，用酶标仪测OD570nm，以确定天然单体化合物是否抑制MRSA生物被膜形成和计算出抑制率，最后筛选出具有抑制MRSA生物被膜形成的单体化合物。根据抑制率，我们认为，来自杂交鹅掌楸的二氯甲烷部位、甲醇部位、正己烷部位、来自白刺的提取物MH-3、MH-6具有抗MRSA生物被膜形成活性，而MH-1、MH-2、LXP-1、LXP-2、LXP-4是否具有抗MRSA活性或抑制活性还有待进一步确认。63770
毕业论文关键词 鹅掌楸 白刺 细菌 耐甲氧西林金黄色葡萄球菌 细菌生物被膜 非经典抗菌
毕业设计说明书（论文）外文摘要
Title The investigation of non-classical antibacterial extracts or molecules from two medicinal plants
Abstract
In this paper, anti-biofilm or biofilm inhibition of some extracts and natural compounds from two medicinal plants were studied with pathogenic bacteria (MRSA). All these screenings were carried out in 96 - well microtiter plates, and crystal violet staining to quantify biofilm, measured at OD570nm with a microplate reader in order to determine whether extracts or natural compounds inhibit MRSA biofilm formation or not, Finally we found a few extract and natural compounds could inhibit MRSA biofilm formation. According to the inhibitory rate, we suggested that three extracts, dichloromethane part, methanol part, n-hexane part, from Liriodendron bybrid, two extracts, MH-3 and MH-6, from Nitraria Tangutorum had anti-biofilm formation activity of MRSA, while whether MH-1, MH-2, LXP-1, LXP-2, LXP-4 had anti-biofilm formation activities or not are still being confirmed.
Keywords Liriodendron hybrid Nitraria tanggurum Bacteria Methicillin-resistant staphylococcus aureus Bacterial biofilm Non-classical anti-bacteria.
1 引言 1
1.1 细菌生物被膜介绍 2
1.1.1 细菌生物被膜的组成成分 2
1.1.2 细菌生物被膜的结构 2
1.1.3 细菌生物被膜的形成过程 3
1.1.4 细菌生物被膜中细菌的生理状态 4
1.2 细菌的群体感应系统 4
1.3 细菌生物被膜可能的耐药机制介绍 5
1.4 耐甲氧西林金黄色葡萄球菌简介 7
2 实验部分 7
2.1 实验材料和实验仪器 7
2.1.1 实验材料 7
2.1.2 实验试剂 8
2.1.3 实验仪器 8
2.2 实验原理 8
2.3 实验步骤 9
2.4实验结果与讨论 11
2.4.1 实验结果 11
2.4.2 实验讨论 18
结 论 20
致 谢 21
参考文献